摘要
目的:观察肝细胞生长因子(hepatocyte growth factor,HGF)作用下,脐血干细胞在体内向肝细胞分化的情况．方法:HGF的裸DNA质粒通过尾静脉快速注入6-8周龄的非肥胖糖尿病／重症联合免疫缺陷(NOD／SCID)小鼠体内,酶联免疫吸附法 (ELISA)检测外周血中HGF水平．收集足月妊娠产妇的脐血,磁式分选法选出CD34+造血干细胞(hematopoietic stem cell,HSC)．20 μL四氯化碳(carbon tetrachloride,CCl4)腹腔注射建立NOD／SCID小鼠急性肝损伤模型,分别输注 CD34+HSC和／或HGF质粒,观察其对死亡率、肝功能恢复的影响,并检测小鼠肝组织内的人源性细胞．结果:快速注入HGF质粒后,体内HGF水平明显上升．实验各组存活率及肝功能恢复情况无明显差异．病理切片显示,联合应用 HGF+HSC的实验组,其肝组织损伤程度最轻, 单独应用HGF或HSC的两个实验组结果类似, 对照组最重．在两组注射HSC的小鼠肝组织中,均可检测到人源性的、分泌白蛋白的肝样细胞,且联合应用HGF的实验组中,此种细胞更多,分布更广．结论:HGF可以促进脐血干细胞向肝细胞分化,并发挥肝细胞功能．
AIM： To observe the hepatocyte growth factorinduced differentiation of CD34^＋ hematopoietic stern cells （HSC） from umbilical cord blood into hepatocyte-like cells.
METHODS： Systemic administration of naked plasmid containing HGF cDNA driven under cytornegalovirus promoter （pCMV-HGF） were injected rapidly via the tail vein of the NOD/ SCID mice, and the level of HGF protein in the peripheral blood was detected by enzyme-linked irnrnunosorbent assay. CD34^＋ human hernatopoietic stern cells were isolated from umbilical cord blood by magnetic cell sorting method. 20 μL CCl4 was administered into the mice to establish the model of acute liver damage and hepatocyte proliferation, pCMV-HGF injection and/or CD34^＋ human hernatopoietic stern cells transplantation were performed on the model mice. Then the mortality of the mice and liver function recovery status were observed. Human specific mRNA and protein were also detected in the mice by reverse transcription polymerase chain reaction and immunohistochemistry, respectively （RT-PCR）.
RESULTS： A remarkable enhancement of human HGF protein level was observed in the peripheral blood of the mice. The mortality and status of liver function were not significantly different between each experiment group. Pathological examination showed that the mice received combined treatment HGF and HSC had the lightest liver injury, while the liver injury was not markedly different between the mice received HGF and HSC alone. Human albumin mRNA and protein were all expressed in the liver tissues underwent HSC transplantation with or without HGF, while in the mice with HGF injection, there were much more hepatocyte-like cells.
CONCLUSION： Stem cells from umbilical cord blood can differentiate into hepatocyte-like cells, and HGF can promote this process.
出处
《世界华人消化杂志》
CAS
北大核心
2006年第8期767-771,共5页
World Chinese Journal of Digestology
关键词
肝细胞生长因子
造血干细胞
肝细胞
Hepatocyte Growth Factor
Hematopoietic Stem Cell
Hepatocyte-like cells
作者简介
范烨，男，1978-09-28生，江苏无锡人，汉族．南京医科大学在读博士，主要研究方向为肝再生、肝移植．
通讯作者：王学浩，210029，江苏省南京市广州路300号．南京医科大学第一附属医院肝脏外科wangxuehao@jsltc．cn 电话：025-83718836-6476
