摘要
目的:观察大鼠无负重力竭游泳后肾脏组织匀浆丙二醛含量、游离巯基含量和钠钾泵腺苷三磷酸酶活性的变化。方法:实验于2001-03/05在华东师范大学体育与健康学院实验室完成。选取清洁级雄性SD大白鼠30只,随机分为4组:正常对照组7只,运动后即刻组7只,运动后1h组8只,运动后24h组8只。①运动后即刻组、运动后1h组、运动后24h组大鼠在玻璃泳池中做无负重的力竭游泳,一般3.5～4.0h。泳池规格1.0m×0.5m×0.5m,水深0.4m,水温32℃。力竭标准为动物下沉后10s不露出水面。正常对照组大鼠不进行任何运动,仅常规饲养。②各组分别于运动后各时相点活杀取材,进行肾脏组织丙二醛含量、游离巯基含量及钠钾泵腺苷三磷酸酶活性的测定。结果:实验选取清洁级雄性SD大鼠30只,全部进入结果分析。力竭游泳后各组大鼠肾脏组织丙二醛含量、游离巯基含量及钠钾泵腺苷三磷酸酶活性的变化:与正常对照组比较,各运动组肾脏组织丙二醛含量均明显升高,且运动后1,24h组尤为显著(P<0.01或0.05);各运动组游离巯基含量、钠钾泵腺苷三磷酸酶活性均明显降低,且运动后1,24h组尤为显著(P<0.01或0.05)。结论:大鼠力竭性游泳运动可导致肾组织匀浆脂质过氧化水平提高,巯基含量下降,钠钾泵腺苷三磷酸酶活性降低,这些可能是运动性疲劳和运动性蛋白尿发生的重要原因之一。
AIM： To observe the changes in contents of malondialdehyde （MDA） and free sulfhydryl （frce-SH） as well as Na^＋-K^＋- pump adenosine triphosphatase （Na^＋-K^＋-ATPase） activity in kidney homogenate of rats after exhaustive swimming without load.
METHODS： The experiment was conducted in the Laboratory of School of Physical Education and Health, East China Normal University from March to May 2001. Thirty male SD rats of clean grade were selected and randomly divided into 4 groups： Normal control group （n=7）, immediate postexercise group （n=7）, 1-hour postexercise group, （n=8） and 24-hour postexercise group （n=8）.①Rats in the immediate postexercise group, 1-hour postexercise group and 24-hour postexercise group were required to conduct exhaustive swimming in glass pool without load for 3.5-4.0 hours. Size of pooI was 1.0 m×0.5 m×0.5 m with the water-depth of 0.4 m and the temperature was 32 ℃. Exhaustive swimming standard was 10 seconds after rats sank into water without outcrop. Rats in the normal control group did no exercise and wrer fed routinely.②Rats in each group were executed at each time point after exercise to determine the contents of MDA and free-SH and the activity of Na^＋-K^＋-ATPase in kidney tissues.
RESULTS： Thirty selected male SD rats of clean grade were involved in the analysis of results. Changes in contents of MDA and frce-SH as well as Na^＋-K^＋-ATPase activity in kidney tissues of rats in all groups after exhaustive swimming： Compared with normal control group, the content of MDA in kidney tissues of each exercise group siguificantly increased, and that in 1-hour and 24-hour postexercise groups were most remarkable （P 〈 0.01 or 0.05）; The content of frce-SH and the activity of Na^＋-K^＋-ATPase in each exercise group were obviously decreased, moreover, those in 1-hour and 24-hour postexercise groups were especially marked （P 〈 0.01 or 0.05）.
CONCLUSION： Exhaustive swimming can lead to an increase of lipid peroxidation in kidney homogenate and a decrease of frce-SH content as well as a reduction of Na^＋-K^＋-ATPase activity, which may be an important reason of sports fatigue and athletic proteinuria.
出处
《中国临床康复》
CSCD
北大核心
2006年第20期113-115,共3页
Chinese Journal of Clinical Rehabilitation
作者简介
李娟，女，1964年生，辽宁省沈阳市人，汉族，2004年沈阳师范大学毕业，硕士，副教授，主要从事运动解剖学方面的研究。
