摘要
目的了解我院大肠埃希菌及肺炎克雷伯菌中AmpC酶产生情况。方法按NCCLS推荐的超广谱β内酰胺酶(ESBLs)确证试验,对大肠埃希菌和肺炎克雷伯菌进行ESBLs检测,并用三维试验法、三维试验抑制试验进行AmpC酶检测。结果236株大肠埃希菌和135株肺炎克雷伯菌中,检到产ESBLs大肠埃希菌104株和产ESBLs肺炎克雷伯菌29株,其中106株耐头孢西丁菌株检到3株产AmpC酶。结论我院这两种细菌主要耐药机制仍是产生ESBLs,耐头孢西丁可能是膜孔蛋白缺失所致。
Objective To detect AmpC 9-1actamases from clinical isolates of escherichia coli and klehsoella pneumoniae in our hospital. Methods Using confirmatory test recommended by the NCCLS to detect ESBLs producing escherichia coil and klebsoella pneumoniae. Adopting three dimensional extract test and three dimensional depression test to detect AmpC β-lactamases. Results Among 236 escherichia coil strains collected, 104 were ESBLs producing strains. Among 135 klebsoella pneumoniae strains collected,29 were ESBLs producing strains. Among 106 cefoxitin-resistant strains,AmpC β-lactamases producing strains were found in 3 strains. Conclusion ESBLs are the most important resistant mechanisms of the two bacteria isolates in our hospital. The strains cefoxitin-resistant may result from the loss of the membrane porin.
出处
《中国基层医药》
CAS
2006年第3期403-404,共2页
Chinese Journal of Primary Medicine and Pharmacy
