摘要
AIM： To investigate the effect of the serum of patients with chronic hepatitis B （CHB） on apoptosis of renal tubular epithelial cells in vitro and to study the role of hepatitis B virus （HBV） and transforming growth factor-β1 （TGF-β1） in the pathogenesis of hepatitis B virus associated glomerulonephritis （HBV-GN）. METHODS： The levels of serum TGF-β1 were measured by specific enzyme linked immunosorbent assay （ELISA） and HBV DNA was tested by polymerase chain reaction （PCR） in 44 patients with CHB ,and 20 healthy persons as the control. The normal human kidney proximal tubular cell （HK-2） was cultured together with the sera of healthy persons, CHB patients with HBV-DNA negative（20 cases） and HBV-DNA positive （24 cases） for up to 72 h. Apoptosis and Fas expression of the HK-2 were detected by flow cytometer. RESULTS： The apoptosis rate and Fas expression of HK-2 cells were significantly higher in HBV DNA positive serum group 19.01±5.85% and 17.58±8.35%, HBV DNA negative serum group 8.12±2.80% and 6.96 ± 2.76% than those in control group 4.25±0.65% and 2.33 ± 1.09%, respectively （P 〈 0.01）. The apoptosis rate and Fas expression of HK-2 in HBV DNA positive serum group was significantly higher than those in HBV DNA negative serum （P 〈 0.01）. Apoptosis rate of HK-2 cells in HBV DNA positive serum group was positively correlated with the level of HBV-DNA （r = 0.657）. The level of serum TGF-β1 in CHB group was 163.05 ± 91.35 μg/L, signifi- cantly higher as compared with 81.40 ± 40.75 μg/L in the control group （P 〈 0.01）.CONCLUSION： The serum of patients with chronic hepatitis B promotes apoptotic damage in human renal tubular cells by triggering a pathway of Fas up-regulation. HBV and TGF-β1 may play important roles in the mechanism of hepatitis B virus associated glomerulonephritis.
瞄准：在肾的管状的上皮细胞在试管内的 apoptosis 上与长期的肝炎 B ( CHB )调查病人的浆液的效果并且在肝炎 B 的致病学习肝炎 B ( HBV )和转变生长因素贝它( 1 )(TGF贝它( 1 ))的角色病毒联系了肾小球性肾炎( HBV-GN )。方法：浆液 TGF 贝它(1 ) 的层次被特定的酶测量连接免疫吸着剂试金(ELISA ) 和 HBV DNA 被聚合酶链反应(PCR ) 作为控制与 CHB，和 20 个健康的人在 44 个病人测试。正常的人的肾近似管状的房间(HK-2 ) 和重量的单位是有教养的一 of 健康的人，有 HBV-DNA negative (20 个案例) 的 CHB 病人和 HBV-DNA 积极(24 个案例) 为多达 72 h。Apoptosis 和 HK-2 的船边交货表示被流动血细胞计数器检测。结果：HK-2 房间的 apoptosis 率和船边交货表达式在 HBV DNA 积极浆液组 19.01%+/-5.85% 和 17.58%+/-8.35% 是显著地更高的， HBV DNA 否定浆液在控制组 4.25%+/-0.65% 和 2.33%+/-1.09% 比那些组织 8.12%+/-2.80% 和 6.96%+/-2.76% ，分别地(P<0.01 ) 。在 HBV DNA 积极浆液组的 HK-2 的 apoptosis 率和船边交货表示比在 HBV DNA 否定浆液(P<0.01 ) 的那些显著地高。在 HBV DNA 积极浆液组的 HK-2 房间的 Apoptosis 率断然与 HBV-DNA 的水平被相关(r = 0.657 ) 。在 CHB 组的浆液 TGF 贝它(1 ) 的水平是 163.05+/-91.35 microg/L，象与在控制组(P<0.01 ) 的 81.40+/-40.75 microg/L 相比显著地更高。结论：有长期的肝炎 B 的病人的浆液由触发船边交货起来规定的一条小径在人的肾的管状的房间支持 apoptotic 损坏。HBV 和 TGF 贝它(1 ) 可以在肝炎 B 的机制起重要作用联系肾小球性肾炎。
基金
Supported by the Applied Basic Research Programs of Science and Technology Commission of Sichuan Province, No. 01SY051-29
作者简介
Correspondence to： Cun-Liang Deng, Department of Infectious Diseases, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, Sichuan Province, China. dengcun164@vip.sina.com Telephone： ＋86-830-2295772 Fax： ＋86-830-2295772Co-Correspondence author： Xin-Wen Song
