摘要
目的:制备不同钇含量的钇/羟基磷灰石(Y/HA)复合纳米晶体微粒并检测其结构、化学和生物学性能。方法:采用X射线衍射法(XRD)进行物相分析,透射电镜(TEM)观察纳米微晶的形貌和尺寸,电感耦合等离子体质谱仪(ICP)检测溶解性,并对其生物学作用进行了检测。结果:Y/HA纳米微晶晶化好,钇元素进入羟基磷灰石晶体结构中并取代部分钙元素,形貌为纳米级针状微晶,平均晶粒大小为长33.4nm,宽为22.9nm～27.5nm。钙和钇的溶出随着钇含量的增大增多;能促进人牙周膜细胞和大鼠成骨细胞生长,对口腔细菌有一定的抑制作用。结论:机械化学水热法合成的钇/羟基磷灰石复合针状纳米微晶,在形态、晶体结构、合成和结晶度方面与生物骨、牙组织的磷灰石相似,具有更好的生物、化学活性及一定的抑菌作用。
Objective： The aim of this study was to synthesize yttrium/hydroxyapatite nanocrystals particles and submit to a structural, chemical and biological characterization. Methods： The syntheses were carried out in the presence of different concentrations of yttrium （ 1mol% ,5mol% ） in solutions by means of hydrothermally syntheitzed. The X- ray diffraction showed that nanoacrystal sructure and phase appeared at different yttrium ratio content. Morphology of nanocystal suspension was observed by transmission electron microscope. The solubility of calcium and yttrium contents were measured by the inductively coupled plasma mass spectrometry. The biological actions were evaluated by the cell cultivation method and oral bacterial colony counting method. Results： Yttrium can be incorporated into the hydroxyapatite structure. On the basis of the values of XRD, it can be suggested that probably just a part of yttrium is substituted for calcium, the remainder being adsorbed on the crystal surface. However, yttrium destabilizes the apatite structure favouring its dissolution of calcium and yttrium contents and displays an inhibiting effect on the crystallization of hydroxyapatite. Sizes of the nanocrystals were less than 100nm. The shape was needle - like. The human periodontal fibroblast and rat osteoblast growth were accelerated and oral bacterial growth was lightly restricted. Conclusion： The data indicate that the hydrothermally synthetized approach can be successfully used to prepare yttrium/hydroxyapatite nanocrystals with crystallinity, crystal dimensions, structure and stability close to those characteristic of biological apatite and with better biological activity, bacteriostasis.
出处
《中国口腔种植学杂志》
2006年第1期1-4,共4页
Chinese Journal of Oral Implantology
关键词
羟基磷灰石
纳米晶体
钇
Hydmxyapatite
Nanocrystals
Yttrium
