摘要
考察了pH值、邻二甲苯–4–磺酸与L–亮氨酸的物质的量比、氯化铵浓度和温度等因素对邻二甲苯–4–磺酸反应沉淀L–亮氨酸的影响。结果表明:邻二甲苯–4–磺酸沉淀L-亮氨酸的适宜pH值为不高于1.5;邻二甲苯–4–磺酸与L-亮氨酸的最适物质的量比为3∶1;溶液中氯化铵浓度增大使L–亮氨酸的沉淀率缓慢下降;温度升高L–亮氨酸的沉淀率迅速降低,反应应于低温下进行,实际生产中可选择在0℃。按上述最佳工艺条件从除酸后猪血粉水解液中提取L–亮氨酸,沉淀率达到90.62%,经精制L-亮氨酸产品达到《中华人民共和国药典》(2000年版二部)的标准。
The effects of pH, temperature, mol ratio of 3,4-dimethyl-benzenesulfonic acid to L-leucine and NH4Cl concentration on the L-leucine precipitation yield by reacting with 3,4-dimethyl-benzenesulfonic acid were investigated. The pH value must be kept lower than 1.5, the optimal mol ratio of 3, 4- dimethyl-benzenesulfonic acid to L-leucine was 3 ：1. A low temperature was favorable for precipitation, preferably 0℃.The yield decreased with the increase of NH4Cl concentration. L-leucine was separated from pig blood hydrolysate after removal of acid, and the precipitation rate was 90.62%. The L-leucine after refinement was in agreement with the standard of Pharmacopoeia of the People＇s Republic of China （2000 edition, the second section）.
出处
《化工进展》
EI
CAS
CSCD
北大核心
2006年第4期439-443,共5页
Chemical Industry and Engineering Progress
基金
国家自然科学基金项目(No.30370415)
福建省科技计划重点项目(2004H013)
泉州市科技计划重点项目(2004G48
2005G6)
泉州市科技计划重大项目子项目(2005ZD4_5)
关键词
邻二甲苯-4-磺酸
L-亮氨酸
沉淀
3, 4-dimethyl-benzenesulfonicacid
precipitation
L-leucine
作者简介
翁连进（1969-）男，博士，副教授。主要从事氨基酸分离提纯研究及生产工艺开发。电话0595-22693828：E-mail ljweng6907@sina．com。
