摘要
目的建立反相高效液相色谱法测定抗感颗粒中绿原酸的含量。方法采用Hypersil C18（250mm×4．6mm，5μm）色谱柱；以乙腈-0．4％磷酸溶液（13：87）为流动相，流速为1．0mL·min^-1，柱温为室温，检测波长为327nm，以外标法测定了金银花药材及抗感颗粒中绿原酸的含量。结果绿原酸在2．24—26．88μg·mL^-1内呈线性关系。绿原酸在制荆中的平均回收率（n=5）为99．3％（RSD=0．99％）。结论该方法准确、简便、重现性好，可用于抗感颗粒的质量控制。
OBJECTIVE To establish the method of determination of chlorogenic acid in Flos Lonicerae and Kanggan granules by RP-HPLC. METHODS An Hypersil C18 column was used with a mobile phase consisted of mixture of acetonitrile-0.4% phosphoric acid （ 13：87 ） at flow rate of 1.0mL · min ^- 1 in this method. And UV detector wavelength was 327nm. RESULTS The linear range of chlorogenic acid was 2.24- 26. 88μg · mL^- 1 （ r = 0. 999 8）. The average recovery was 99.3% （ RSD = 0.99%, n = 5 ）. CONCLUSION An accurate, simple, rapid qualitative method with good reproducibility to assay chlorogenic acid in Flos Lonicerae raw material and Kanggan granules is established.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2006年第2期160-162,共3页
Chinese Journal of Modern Applied Pharmacy
