摘要
用免疫组织化学方法检测原癌基因cf-os和c-myc蛋白在北方山溪鲵(Batrachuperus tibetanus)精子发生中的表达定位。结果显示,在精原细胞缓慢增殖期,8、9月,FOS阳性反应物出现在精原细胞的胞质及核膜外,10、11月,FOS在少量精原细胞的胞核中表达。在精原细胞快速增殖期,即翌年4月,FOS定位在精原细胞的胞质中;5月,FOS在大量的胞核中强阳性表达;6月,FOS定位于部分精母细胞核质和核膜下;7月,FOS在一些精子细胞的核质和核膜下表达。MYC在8、9月的部分精原细胞胞质中表达较弱,在101、1月阳性反应出现在个别精原细胞的核质中。翌年4月,MYC在精原细胞核周围的胞质中表达;5月在大量的精原细胞核膜下有强表达;6月,MYC在一些精母细胞核膜下表达;7月,MYC在部分精子细胞的核膜下弱表达。结果表明,北方山溪鲵的原癌基因cf-os和c-myc表达大强度在生精细胞发育中呈阶段性,表达的强度和细胞数量与细胞增殖的速度相一致。FOS和MYC在精原细胞内从胞质向胞核的转移与细胞快速增殖的时期相吻合。说明cf-os和c-myc对精原细胞有丝分裂有促进作用,并参与精母细胞成熟分裂的调控。
The expression of proto-oncogene c-fos and c-myc has been studied by immunocytochemistry in the testis of the Stream Salamander （ Batrachuperus tibetanus ）. During slow proliferation of spermatogonia, FOS protein was detected in cytoplasm or pefinuclear region of spermatogonia in August and September, and positive reactivity presented in the nuclei of a few spermatogonia in October and November. During quick proliferation of spermatogonia, FOS was expressed in the cytoplasm of spermatogonia next April, and strong positive reactivity presented in the nuclei of spermatogonia in May. In June, FOS appeared in nucleoplasm and nuclear membrane in some spermatocytes, while they appeared in nucleoplasm and nuclear membrane of spermatids in July. In August and September, there was slight immunoreactivity for MYC in the cytoplasm of some spermatogonia, and they were detected in nuclei of spermatogonia in October and November. In the next April, MYC was expressed in cytoplasm around the nuclei of spermatogonia, and positive reactivity presented in more spermatogonia nuclei in May. MYC appeared in the nuclear membrane of some spermatocytes in June, and there was a slight immunoreactivity in the nuclear membrane of spermatids in July.The results show that expression of proto-oncogenes c-fos and c-myc during the development of spermatogenic cells is in a stage-specific manner in B. tibetanus, and that the quantity and intensity of cells in which proto-oncogenes are expressed are correlated with the speed of cell proliferation. Translocation of FOS and MYC from cytoplasm to nuclei of spermatogonia is consistent with quick proliferation of spermatogonia. It can be concluded that proto-oncogenes c-fos and c-myc may function to promote mitosis of spermatogonia and to regulate meiosis of spermatocytes.
出处
《动物学杂志》
CAS
CSCD
北大核心
2006年第2期29-36,共8页
Chinese Journal of Zoology
基金
陕西省自然科学研究计划项目(No.2001SM26)
关键词
北方山溪鲵
精子发生
C-FOS
C-MYC
Stream Salamander （ Batrachuperus tibetanus ）
Spermatogenesis
c-fos
c-myc
作者简介
李亚琳，女，博士研究生；研究方向：动物生殖内分泌。通讯作者，E-mail：yu—huizhang@163．com；
