摘要
目的观察骨关节炎(OA)和类风湿关节炎(RA)患者滑液对中性粒细胞凋亡的影响。方法通过流式细胞仪及荧光显微镜检查19例RA和11例OA患者对体外培养粒细胞凋亡的影响。结果流式细胞仪及荧光显微镜检测的体外培养粒细胞自发凋亡发生率分别为43.6%和37.4%,加RA滑液组分别为18.3%和13.7%(P<0.01),加OA滑液组则分别为40.3%和31.2%(P>0.05)。结论RA滑液可显著降低粒细胞凋亡发生率,流式细胞仪检测凋亡较荧光显微镜检查更具敏感性。
Objective To investigate neutrophil apoptosis in the presence of synovial fluids （SF） from patients with rheumatoid arthritis （RA）and osteoarthritis（OA）. Methods Neutrophil apoptosis was determined by flow cytometric analysis of annexin-Ⅴ binding,and by terminal transferase-mediated dUTP-fluorescensin nick end-labeling （TUNEL） technique in the presence or absence of synovial fluids from 19 RA patients and 11 OA patients . Results In the presence of RA SF,the rate of neutrophil apoptosis was reduced from 43. （3% to 18.3% （P〈0. 01） as detected by flow cytometric analysis ; and from 37.4% to 13.7% （P〈0. 01） as assayed by TUNEL method. Whereas,no significant change of neutrophil apoptosis was observed in the persence of OA SF. Conclusion This study indicates that RA SF is capable of slowing the rate of neutrophil apoptosis,and that flow cytometric analysis of annexin-Ⅴ binding is more sensitive than TUNEL method in detection of cell apoptosis.
出处
《现代检验医学杂志》
CAS
2006年第2期41-43,共3页
Journal of Modern Laboratory Medicine
关键词
流式细胞术
关节炎
类风湿
细胞凋亡
flow cytometric analysis
rheumatoid arthritis
osteoarthritis apoptosis
作者简介
杨明辉（1967-）,男,大学文化,主管技师,参与主研的成果＂类风湿关节炎发病机制及临床研究＂、＂骨关节炎基础与临床研究＂分获2003年和2005年四川省科技进步一等奖.目前主要从事风湿免疫性疾病的研究,联系电话：0817-2262325,E- mail：ymh6782003@yahoo.com.cn.
