摘要
从南极普里兹湾深海沉积物中筛选到一株产低温脂肪酶的菌株7195,细菌学形态鉴定及16SrDNA序列分析表明该菌株属于嗜冷杆菌属(Psychrobacter)。生长特性研究表明该菌株属于耐冷菌,其最适生长温度范围为5～15°C,7195菌株能利用多种碳、氮源产酶。粗酶液经硫酸铵盐析、DEAEcellulose-52柱层析进行初步分离纯化后进行酶学性质的研究。该菌株所分泌的脂肪酶最适作用温度为30°C,最适pH值为9.0,对热敏感,60°C热处理10min剩余酶活为30%,是典型的低温酶。Ca2+、Mn2+、Cu2+对该酶有较为明显的激活作用,而Co2+、Zn2+、Hg2+、Rb2+、Cd2+、Fe3+、EDTA则能抑制酶活,此外该脂肪酶能在高浓度的SDS、CHAPS、TritonX-100、Tween80、Tween20等变性剂中表现出较好的稳定性。
A strain 7195, which produces cold - adapted lipase, was isolated from the deep sea sediment of Prydz Bay, Antarctic. The morphology identification and 16S rDNA sequence analysis showed flint it belonged to genus Psyehrobaeter. The optimal growth temperature of the Psychrobaeter sp. 7195 was 5 ～ 15℃, indicating it was a psychrophile bacterianl. The strain could utilize various single earbonaceous and nitrogenous substances and produce lipase. The optinal and highest temperatures for enzyme producing were 20℃ and 30℃ respectively. The enzyme purification was performed by ammonium sulfate fractionation and anion exchange chromatography with DEAE cellulose - 52. It was sensitive to high temperature. The optimal temperature and pH value for the lipase activity were 30℃ and 9.0 respectively. These results indicated flint they were typical alkaline cold - adapted enzymes. The activities of them were stimulated by Ca^2＋ , Mn^2＋ , Cu^2＋ and were inhibited by Zn^2＋、 Hg^2＋ ,Rb^2＋ ,Co^2＋ , Co^2＋ , Fe^3＋ and EDTA. The cold- adapted lipase showed better resistance to inactivation of 1% detergents such as SDS, CHAPS, etc.
出处
《生物磁学》
2006年第1期6-10,共5页
Biomagnetism
基金
国家自然科学青年基金(No.40406029)~~
关键词
低温酶
碱性脂肪酶
耐冷菌
南极
Cold- adapted enzyme
Alkaline lipase
Psychrophile bacteria
Antarctic
作者简介
张金伟,在读硕士研究生,E-mail： jinweizhang@21cn.com
曾润颖,通讯作者,E-mail： runyingzeng@yahoo.com.cn
