摘要
采用生态学单因子梯度试验方法,研究了水环境中添加0·01、0·10、1·00、5·00mg/L的铜(Cu2+)对中华绒螯蟹肝胰腺、血淋巴和鳃代谢酶活力的影响。结果表明,随着Cu2+浓度的增高,肝胰腺和血淋巴的铜锌超氧化物歧化酶(Cu-Zn SOD)、酸性磷酸酶(ACP)、碱性磷酸酶(AKP)活力和血蓝蛋白均呈先增加后减少的变化趋势。鳃钠钾三磷酸腺苷酶(Na+,K+-ATPase)、钙镁三磷酸腺苷酶(Ca2+,Mg2+-ATPase)和谷丙转氨酶(GPT)活力均随着Cu2+浓度的增高而降低。随着Cu2+浓度的增高,肝胰腺髓性过氧化物酶(MPO)活力呈先减少后增加的变化趋势。水环境中添加0·01mg/L Cu2+就可对中华绒螯蟹代谢酶产生显著影响,并随着Cu2+浓度的增高,对中华绒螯蟹机体的影响逐渐加剧。表明代谢酶活力的变化可以灵敏地反映Cu2+的胁迫程度和毒性。
Effect of water-borne copper Cu^2＋ in different concentrations （0.00, 0.01, 0. 10, 1.00, 5.00mg/L） on activities of several main metabolic enzymes in hepatopancreas, hemolymph and gill of Eriocheir sinensis were studied by Single Gradient Factor Experiments. The result showed early rise-up and later fall-down Curve in Cu-Zn SOD activity in hepatopancreas, with increasing of Cu^2＋ level and the turn-over point of 26. 018U/mg at 1.00mg/L Cu^2＋ The maximum activities of ACP （170. 861U/mg） and AKP （267. 238U/mg） were observed at 0. 10mg/L Cu^2＋ , while the minimum activities of ACP （ 123. 879U/mg） and AKP （ 133. 244U/mg） were at 5.00mg/L Cu^2＋ . The activity of MPO was significantly different between the controls and treated groups when Cu^2＋ level rises. The lowest （1. 605U/g） and highest value （8. 743U/g） of the enzyme were at the water-borne of 0.01mg/L Cu^2＋ and 5.00mg/L Cu^2＋ , respectively. Gill impairment by Na^＋, K^＋ -ATPase and Ca^2＋ , Mg^2＋ -ATPase activities occurred when exposed to water-borne Cu^2＋ , obtaining the highest and lowest value in the controls and 5.00mg/L Cu^2＋ treated group, respectively. In hemolymph the activity of Cu-Zn SOD increased in the beginning and then decreased when Cu^2＋ concentration increased, highest （229. 047U/ml） activities at 1.00mg/L Cu^2＋ and lowest （ 199. 539U/ml） at 5.00mg/L Cu^2＋. The highest activity of GPT （38. 132U/L） was obtained in the controls, while the lowest one （0.024U/L） was observed at 5.00mg/L Cu^2＋ group. Increasing Cu^2＋ level was also associated with the activity of ACP, showing early increase at the peak of 3. 529U/100ml and later decrease being at the trough of 2. 171U/100ml corresponding to 0.10 and 5.00mg/L Cu^2＋ , respectively. Similarly the activity of AKP peaked to 2. 496U/100ml at 0.10mg/L Cu^2＋ and dipped to 1. 642U/100ml at 1.00mg/L Cu^2＋ In addition, hemocyanin activity in the hemolymph exhibited a trend of early up and later down, 485. 227U/L the maximum at 0. 10mg/L Cu^2＋ and the minimum 89. 200U/L at 5.00mg/L Cu^2＋ .
It should be emphasized that a significant impairment to metabolism enzymes can occur even at 0. 01mg/L Cu^2＋ , a very low level that is in the range of National Water Quality Standard for Fisheries （GB11607 -89）. The changes in the activities of enzyme can be used as a sensitive biomarker to Cu^2＋ toxicity for E. sinensis. More species-specified national water quality standards are suggested to be applied to different economic animals.
出处
《海洋与湖沼》
CAS
CSCD
北大核心
2006年第2期118-124,共7页
Oceanologia Et Limnologia Sinica
基金
中国水产科学研究院首席科学家资助金项目
1-105011号
关键词
中华绒螫蟹
肝胰腺
血淋巴
鳃
代谢酶
铜
Eriocheir sinensis, Hepatopancreas, Hemolymph, Gill, Metabolism enzyme, Copper
作者简介
杨志彪，助理研究员，博士研究生，E-mail：nbuyzb@163.com.
通讯作者：杨健，研究员，E-mail：jiany@ffrc．ac
