摘要
在对人工栽培的富硒蛹虫草中分离提取了水溶性硒多糖,富硒蛹虫草硒多糖的分离纯化方法是采用水提醇沉工艺,粗硒多糖得率为5.76%。系统研究了富硒蛹虫草硒多糖的浸提、纯化、分离条件,苯酚硫酸法测定了富硒蛹虫草的多糖含量为47.5%,分子荧光法测定硒多糖中含硒量为92.3mg.kg-1。
In the present paper, the water soluble selenium polysaccharide was extracted, and isolated and purified preliminarily. The traditional method was used to extract selenium polysaccharide, which was extracted by water and sank by ethanol. The work also focused on the determination of the experiment conditions, optimizing the experiment conditions such as proportion of extract solution, extract temperature, extract times, proportion of ethanol, and standing time. The recovery of selenium polysaccharide under these conditions is 5.76%. Polysaccharide content in the powder is 46.6%, and selenium content is 92.3 mg · kg^-1.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2006年第3期522-525,共4页
Spectroscopy and Spectral Analysis
基金
国家自然科学基金(20271024)资助项目
辽宁省科学技术计划项目-省院校合作工程资金专项计划(200502003)
关键词
硒
富硒蛹虫草
硒多糖
分离
荧光光度法
Selenium
Enriched-Se cordyceps militaris, Selenium polysaccharide
Separation
Fluorescence spectrophotometry
作者简介
邓桂春，女，1952年生，辽宁大学化学科学与工程学院教授。
通讯联系人。
