摘要
目的:探讨延肾胶囊抑制5/6肾切除大鼠残肾纤维化、延缓慢性肾衰竭的作用机制。方法:取健康Wistar大鼠30只,随机分成假手术对照组、手术组和延肾胶囊治疗组。采用生物化学、形态计量、免疫组化技术检测各组大鼠术后60 d肾功能、肾组织形态学、肾组织蛋白酶B(CB)和Ⅳ型胶原表达。结果:延肾胶囊治疗组比手术组血清肌酐、尿素氮水平明显下降,肾小球硬化程度明显减轻;免疫组化显示,治疗组比手术组大鼠CB表达增多,Ⅳ型胶原表达减少。结论:延肾胶囊可能通过促进CB表达增加,降解Ⅳ型胶原等细胞外基质而抑制5/6肾切除大鼠残肾组织纤维化。
Objective：To explore the effects and mechanism of Yanshen Capsule on the remnant kidney. Methods： Thirty healthy Wistar rats were randomly divided into 3 groups： control group rats were sham - operated and gavaged with saline daily, operation group rats were 5/6 nephrectomized and garaged with saline daily, treatment group rats were 5/6 nephrectomized and garaged with Yanshen Capsule daily. The body weight（BW）, blood urea nitrogen（BUN） and ereatinine （Scr） levels, renal morphological changes, the expression of collagen Ⅳ （Col Ⅳ ） and cathepsin B （CB） were observed using biochemistry, morphometry and immunohistochemistry at 60 days after operation. Results： The BUN and Scr of Yanshen Capsule- treated group also slightly higher than that of control group, but were significantly lower than that of operation group（P 〈 0.01 ）. In Yartshen Catxsule- treated group, lower ratio of sclerosis glomeruli, higher exprssion of CB and lower expression of Col Ⅳ were observed compared to that of operation group. Conelusion： Yanshen Capsule retards the development of renal fibrosis through increasing CB and suppressing Col Ⅳ.
出处
《中国中西医结合肾病杂志》
2006年第3期143-145,共3页
Chinese Journal of Integrated Traditional and Western Nephrology
作者简介
通讯作者
