摘要
采用油水乳化法,以环己烷和碳酸钙微粒复合致孔,以6%的琼脂糖凝胶包裹玻璃珠,制备出大孔薄层琼脂糖凝胶-玻璃珠复合介质,其密度为1.7g.ml-1,平均粒径为127.8μm,在其上接枝二乙胺基乙基制备成阴离子交换介质,进行了牛血清白蛋白的吸附实验.对比实验结果表明此大孔薄层介质具有较好的吸附容量和良好的传质特性.
A macro-porous pellicular agarose glass composite matrix （MPAG） was prepared by coating 6% agarose gel onto glass beads by water-in- oil emulsification using a mixture of cyclohexane and fine calcium carbonate powder as porogenic diluents. The density of MPAG was 1.7 g · ml^-1 and the average bead size was 127.8 μm, MPAG was derivatized with diethylaminoethyl （DEAE） and subjected to the adsorption of bovine serum albumin. Compared to macro porous matrix or homogeneous matrix derivatized with DEAE, DEAD-MPAG gave a higher adsorption capacity at a faster mass transfer rate.
出处
《化工学报》
EI
CAS
CSCD
北大核心
2006年第2期347-352,共6页
CIESC Journal
关键词
色谱
复合介质
琼脂糖凝胶
吸附
传质
chromatography
composite matrix
agarose gel
protein adsorption
mass transfer
作者简介
周鑫（1971-），男，博士．
