摘要
目的：建立反相高效液相色谱法测定人血浆中埃索美拉唑（esomeprazole，EMZ）浓度。方法：血浆样品用二氯甲烷萃取后经YMCC18（150mm×4．6mm，5μm）色谱柱（柱温：35℃），用流动相甲醇-0．02mol·L^-1醋酸铵（NH3·H2O调pH7．6）=51：49，以流速1ml·min^-1洗脱分离，用卡马西平为内标，检测波长306nm。结果：标准曲线线性范围3．9—2000．0ng·ml^-1；萃取回收率70．6％～76．7％；加样回收率89．1％-104．6％；日内测定RSD为4．8％。6．6％，日间测定RSD为2.8％。5．6％。结论：本方法快速、灵敏、准确、简便，适用于EMZ血药浓度测定及药代动力学研究。
Objective： The aim of this study was to develop a RP- HPLC method to determine the concentration of esomeprazole （EMZ） in human plasma. Methods： The plasma sample was extracted with 3ml dichloromethane, then separation was achieved on the YMC Cls （150 × 4.6mm, 5μn） column. The mobile phase consisted of MeOH： 0.02mol/L NH4Ac （Adjust pH with NH3·H2O to 7.6） =51：49 and the effluent was measured at 306 rim. Carbamazepine was used as an internal standard. Results： The retention times for esomeprazole and Carbarnazepine were 11.6min and 9.6min. The calibration curves were linear in the range of 3.9 - 2000.0ng· ml^-1. The quantitation limit of detection in plasma was 3.9ng· ml^-1 ; the extraction recovery of esomeprazole were 70.6-76.7%; the methodological recovery were 89.1 - 104.6%; within - day RSD were 4.82 - 6.57% and interday RSD 2.8 - 5.6%. Conclusion： The assay was simple, rapid, and sensitive. It was satisfied to be used to study the pharmacokinetics and relative bioavailability of esomeprazole in human plasma.
出处
《华西医学》
CAS
2006年第1期93-95,共3页
West China Medical Journal
作者简介
余勤（1969-），女，四川雅安人，主管技师。
通讯作者
