摘要
应用酶联免疫吸附法(enzyme_linkedimmunosorbentassay,ELISA)研究了在褐飞虱胁迫下镇稻2号(粳稻)和协优63(籼稻)抽穗期和灌浆期水稻叶片和根中玉米素核苷(zeatinriboside,ZR)含量变化情况。结果表明,镇稻2号抽穗期ZR含量变化比灌浆期对褐飞虱侵害更为敏感,密度分别为15、30、60、120头株的褐飞虱侵害水稻抽穗期3、6、9天后,叶片和根中ZR含量显著下降;灌浆期除各个褐飞虱密度侵害9天叶片中ZR含量和120头株褐飞虱密度侵害9天根部ZR含量显著下降外,其他处理根和叶片中ZR含量下降不明显。协优63抽穗期受褐飞虱侵害后体内ZR的变化不同于镇稻2号,密度分别为15、30、60头株的褐飞虱侵害3天后,叶片中ZR含量明显升高;在灌浆期,除30、60、120头株褐飞虱密度侵害6天和120头株褐飞虱密度侵害9天叶片中ZR含量有显著增加外,其他处理变化不明显。表明不同品种水稻在不同密度褐飞虱侵害下对根和叶片中ZR含量有不同的影响。
The response of zeatin ribosides （ZRs） in roots and leaves at different developmental stages of two representative rice varieties to N. lugens nymph infestation was studied using enzyme-linked immunosorbent assay （ELISA）. The results indicated that ZR content at the heading stage of Zhendao 2 was more sensitive to N. lugens infestation compared with that at the grain filling stage, with significant reduction of ZR contents in roots and leaves at 3, 6 and 9 days after 15, 30, 60 and 120 nymphs/plant infestations compared with the control. For the grain-filling stage of Zhendao 2, N. lugens infestation did not result in significant reduction of ZR content in roots and leaves except ZR contents in leaves at 9 days after 15, 30, 60, 120 nymphs/plant infestation and ZR content in roots at 9 days after 120 nymph/plant infestations. However, the response of ZR in Xieyou 63 to N. lugens stress was different from Zhendao 2： ZR contents in leaves at 3 days after 15, 30 and 60 nymph infestation increased significantly at the heading stage, and there were no significant alterations of ZR content at the grain filling except with significant increase of ZR contents in leaves at 6 days after 30, 60, 120 nymphs/plant infestation and at 9 days after 120 nymphs/plant infestation. The results demonstrated that the effect of N. lugens infestation on ZR content varied with rice variety and developmental stage.
出处
《昆虫学报》
CAS
CSCD
北大核心
2006年第1期86-92,共7页
Acta Entomologica Sinica
基金
国家"十五"攻关项目(2004BA516A02)
国家自然科学基金资助项目(30070122)
作者简介
刘井兰，女，1974年生，博士研究生，讲师，主要从事害虫综合治理及农药环境毒理研究
通讯作者Tel．：0514-7979246；E-mail：jc．wu@public．yz．js．cn
