摘要
目的分析国内83株布鲁杆菌基因组的插入序列(IS)IS711的遗传多态性。方法提取布鲁杆菌DNA,经内切酶EcoRI酶切、琼脂糖凝胶电泳、Southern转印后,与地高辛标记的IS711探针杂交,以酶免疫试验检测信号,比较布鲁杆菌各株的IS711指纹图谱。结果不同布鲁杆菌菌株在IS711探针杂交图谱上有明显的多态性,根据杂交的条带数及片段大小的差异,83株布鲁杆菌分属15个IS型。以IS10型分布最为广泛,其次是IS3型,其他型别则较分散,有的IS型只存在于某个菌株。结论布鲁杆菌插入序列IS711在基因分布上存在多态性。
Objective To investigate the genetic polymorphism of insert sequence （IS） among 14 Brucella reference strains and 69 field strains isolated in China. Methods Chromosomal DNA of Brucella was extracted and digested with EcoR I. Then it was separated on agarose gel electrophoresis. At last it was transferred into a nylon filter and hybridized with IS711 which was labeled using random primer with digoxigenin-dUTP. Hybrids were detected by enzyme immunoassay and the IS711 fingerprints of different strains were compared. Results IS711 fingerprints of different Brucella strains showed high polymorphism. 83 Brucella strains detected belonged to 15 IS711 types according to the numbers and locations of straps of IS711 fingerprint. IS10 was the most predominant subtype. Other IS711 types were relatively dispersed, among which one type was only found in a certain strain. Conclusions There was high genetic polymorphism with insert sequence 711 of Brucella.
出处
《中国地方病学杂志》
CAS
CSCD
北大核心
2006年第1期42-45,共4页
Chinese Jouranl of Endemiology
基金
国家"973"资助项目(2002CB513206)
作者简介
骆利敏（1978-），女，江苏省连云港市人，博士研究生．主治医师．从事基因工程疫苗研究工作
通信作者：李明（Tel：020-85148321）
