摘要
目的 探讨黄芩素对胃癌细胞凋亡的影响。方法 MTT法测定黄芩素对胃癌SGC-7901细胞生长抑制率。TUNEL染色、AO/EB荧光染色测定细胞凋亡率。结果 胃癌细胞呈典型的凋亡形态学改变,凋亡比例呈剂量时间依赖性。TUNEL染色法显示,细胞凋亡指数2.696~16、196。结论 黄芩素具有诱导胃癌细胞凋亡的作用。
Objective To explore the effect of selective lipoxygenase inhibitor baiealein on cell apotosis of human gastric cancer line SGC-7901. Methods MTT assay was used to determine the cell inhibitory rate. The proportion of apoptosis cells detected by TUNEL staining and acridine orange and ethidium bromides (AO/EB) stains. Results After exposure to the drug, SGC-7901 exhibited morphologie features of apoptosis. The apoptie index was (2.6 ±16.1) % by TUNEL assay. Conclusion Baiealein can induce apoptosis of gastric cancer cells.
出处
《福建医科大学学报》
2006年第1期35-36,43,共3页
Journal of Fujian Medical University
关键词
胃肿瘤
脂氧合酶抑制剂
凋亡
细胞
培养的
stomach neoplasms
lipoxygenase inhibiton
apoptosis
tumor cells, cultured
作者简介
谢建伟(1979~),男,福建医科大学2002级硕士研究生