摘要
目的观察1800MHz电磁波暴露对大鼠海马内NMDA受体亚单位NR2A、NR2B表达的影响。方法将4周龄雌性Wistar大鼠随机分为4组(0.5和1.0mW/cm2剂量组,各实验组分别设置1个对照组),每组12只。其中2个实验组大鼠每天12h暴露于频率为1800MHz、功率密度分别为0.5mW/cm2和1.0mW/cm2的电磁波环境中,共暴露21d,同时,采用免疫组织化学和图像处理方法分析海马CA1、CA3、齿状回NR2A和NR2B的表达。结果(1)NR2A蛋白表达0.5mW/cm2剂量组在CA3区表达的免疫反应灰度值为(8.5±1.5),与对照组的(11.1±1.8)比较,差异有统计学意义;在CA1区和齿状回表达的免疫反应灰度值与对照组比较,差异无统计学意义。1.0mW/cm2剂量组在CA1和CA3区表达的免疫反应灰度值分别为(7.9±1.6)和(8.4±1.7),与对照组的(9.7±1.5)和(11.1±1.8)比较,差异有统计学意义;在齿状回表达的免疫反应灰度值差异无统计学意义。(2)NR2B蛋白表达0.5mW/cm2剂量组在CA1和CA3区表达的免疫反应灰度值分别为(16.4±1.0)和(9.6±1.9),与对照组的(17.8±1.6)和(11.2±2.1)比较,差异有统计学意义;在齿状回表达的免疫反应灰度值差异无统计学意义。1.0mW/cm2剂量组在CA1、CA3和齿状回表达的免疫反应灰度值分别为(13.1±2.4)、(9.3±1.4)和(7.3±0.1),与对照组的(17.8±1.6)、(11.2±2.1)和(8.5±1.0)比较,均差异有统计学意义。结论在本实验条件下,1800MHz电磁波暴露可影响大鼠海马NMDA受体的表达。
Objective To identify the effects of NMDA receptor subunits NR2A and NR2B expression in rat＇ s hippocampus after exposure to 1800 MHz radiofrequency radiation. Methods Fourweek old female Wistar rats were randomly divided into four groups, with 12 animals for each. The subjects in two experimental groups had been continuously exposed to 1800 MHz microwave radiation （CW） with respective power density of 0.5 mW/cm^2 and 1.0 mW/cm^2 12 hours each day for 21 days. Meanwhile, sham-controls were carried out. The brain tissue sections were performed by immunohistochemistry to demonstrate both expressions of NR2A, NR2B immune-activity in the hippocampal CA1, CA3 and DG by using computer-assisted image analysis system. Results In NR2A： the expression of 0. 5 mW/cm^2 power density group was significantly lower than 0 mW/cm^2 power density group in CA3 [ （ 8. 5 ± 1.5 ） vs （ 11.1 ± 1.8）, P〈0. 01]and had not been significantly changed in CA1 and DG. The expression of 1.0 mW/cm^2 power density group was significantly lower than 0 mW/cm^2 power density group in CA1 and CA3 [ （7. 9 ± 1.6） vs （9.7 ± 1.5 ） ; （8.4 ± 1.7） vs （ 11.1 ± 1.8 ）, respective P 〈 0. 05, P 〈 0. 01 ] and had not been significantly changed in DG. In NR2B ：the expression of 0. 5 mW/cm^2 power density group was significantly lower than 0 mW/cm^2 power density group in CA1 and CA3 [ （ 16. 4 ± 1.0） vs （ 17. 8 ± 1.6） ; （9. 6 ± 1.9） vs （11.2 ± 2. 1 ）, respective P 〈 0.05 ]. The expression of 1.0 mW/cm^2 power density group was significantly lower than 0 mW/cm^2 power density group in CA1, CA3 and DG [ （ 13.1 ±2. 4） vs （ 17. 8 ±1.6） ; （9.3 ±1.4） vs （11.2±2. 1） ; （7.3 ±0. 1） vs （8.5 ±1.0）, respective P〈0.01, P〈0.05, P〈 0. 05 ]. Conclusion There were findings of the effects on NMDA receptor subunits in different hippoeampus sections after exposure to 1800 MHz radiofrequeney radiation.
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
2006年第1期21-24,共4页
Chinese Journal of Preventive Medicine
基金
国家自然科学基金资助项目(30640116)
关键词
辐射
大鼠
海马
Radiation
Rats
Hippocampus
作者简介
通信作者：吴锡南
