摘要
目的:构建一种纳米材料的新型酶电极。方法:将酪氨酸酶固定在纳米金修饰的金电极表面,用电化学分析法在乙腈介质中对其组装过程以及活性进行了表征。结果:基于构建的新型酶电极,对儿茶酚的催化响应显示了良好的线性关系,该酶电极最大响应电流密度为23.6μA/cm2,米氏常数为4.28 mm o l/L。结论:该酶电极在乙腈介质中测定儿茶酚,显示良好灵敏度和稳定性。
Objective. To develop new types enzyme electrodes for nonaqueous medium. Methods： A new strategy for immobilization of tyrosinase on the surface of gold nanoparticles which are co-immobilized at a gold disk electrode through thiol-acetic acid molecule by assembly process is demonstrated. A conventional three-electrode system is fabricated to study electrochemical and enzymatic reaction between tyrosinase in the gold nanoparticles and catechol in acetonitrile. The enzymatic activity of tyrosinase in the gold nanoparticles to the above catechol in acetonitrile was confirmed by voltammetry and amperometry. Result： The current density was 23.6μA/cm^2 and the Michaelis constants was 4. 28 mmol/L. Conclusion： Compared with normal Tyro/Au electrode, the analytical property of Tyro/NG/S/Au electrode is improved.
出处
《广西医科大学学报》
CAS
北大核心
2005年第6期860-862,共3页
Journal of Guangxi Medical University
基金
广西自然科学基金资助(桂科基0342052)
桂林市科技攻关项目(20040104-4)
广西科技攻关项目(科技攻关0428008-4J)
关键词
乙腈
酪氨酸酶
纳米金
电化学
acetonitrile
tyrosinase
gold nanoparticles
electrochemical
