摘要
目的研究利福平对异烟肼在犬体内药动学的影响。方法采用自身对照法双周期两制剂交叉设计实验方案,犬取血前3 d连续给予较大剂量的异烟肼片和利福平片,然后A组3只犬饲喂异烟肼2片(200 mg),B组3只犬饲喂异烟肼2片(200 mg)和利福平2片(300 mg),取血样进行测定[色谱柱:ZORBAX SB-ODS柱(4.6 mm×250 mm,5μm);柱温:40℃;流速:1.0 mL.m in-1;进样量:20μL;流动相:甲醇-0.02 mol.L-1磷酸缓冲液(体积分数为2.5%,pH值为6.2);紫外检测(λ=268 nm);以吡嗪酰胺为内标测定异烟肼的含量]。间隔两周后,A、B两组交叉给药,重复试验。结果异烟肼在犬体内代谢的药-时曲线下面积(AUC0→24)在不同犬间的差异有极显著性,异烟肼在不同个体间吸收与消除具明显的个体差异,但不同给药周期间差异无显著性。结论利福平和异烟肼无药物间不良交互影响,利福平对异烟肼在犬体内的药动学无明显影响。
Objective To study the effect of rifampicin on the pharmacokinetics of isoniazid in dogs. Methods A two-period, two-preparation cross-over, auto-control design was employed for the experimental plan. 6 adult dogs were randomly divided into 2 equal groups ： A and B. Dogs of group A were given each 100 mg of isoniazid at 8 a. m. and 8 p. m. , respectively. Dogs of group B were given each 100 mg of isoniasid at 8 a. m. , 300 mg of rifampiein and 100 mg of isonizid at 8 p. m. , respectively. The drugs were administered by gastrogavage and the course of medication lasted 3 days. Dogs of group A were then given each 200 mg of isoniazid while dogs of group B were given 200 mg of isoniazid and 300 mg of rifampicin after a 12 h fasting. Blood samples were taken from the animals for the determination of the plasma concentration of isoniazid with HPLC. A ZORABX SB-ODS column （4.6 mm× 250 mm,5μm） served as the solid phase; the mobile phase was methanol-0.02 mol·L^-1 phosphate butter（pH= 6.2）, column temperature was 40℃ and the flow rate , 1.0 mL· min^-1, amount of sample added, 20μL, ultraviolet detection wavelength, λ= 268 nm, The content of isoniazid was determined with pigrazinamide as the internal standard. After an interval of 2 weeks, dogs of the 2 groups were subjected to a cross-over medication with the method described above, and the afore mentioned laboratory examinations were carried out once more. Results The differences between the AUC0→24 of isoniazid in different dogs were highly significant, indicating apparent diversities of the absorption and elimination of isoniazid in different individuals. The differences between different medication periods, however, were not significant. Conclusion It was shown that rifampicin and isoniazid had no adverse interaction and rifampicin had no evident influence on the phamacokineties of isoniazid in dogs.
出处
《医药导报》
CAS
2006年第2期109-111,共3页
Herald of Medicine
作者简介
付翔（1970-），男，广西临桂人，主管药师，学士，主要从事医院药学工作。电话：0773—2812651，E-mail：fuyanhua70219@21cn．com。
