摘要
本文研究建立了高效液相色谱测定油菜叶片中硫甙总量与分量的测定方法.油菜叶片经20h冰冻干燥,用70%甲醇为提取溶剂,超声波提取8min,1mg硫酸酯酶脱硫酸根,离子交换树脂纯化后,应用高效液相色谱进行油菜叶片硫甙组分定性、定量.该方法加标回收率为94%～103%,样品极差为2.29μmol/g,符合ISO9167国际标准要求.
HPLC was applied to analyze the profiles and contents of glucosinolates which were extracted from Brassica leaves with 70% methanol solvent by ultrasonic methods after freeze-drying. Results showed that the recoveries ranged from 94% to 103% , the range was 2. 29μmol/g, which accorded with ISO9167 standard. The method was precise, simple and practical for leave glucosinolate determination. It could be widely used in quality and diseases resistance breeding for analysis of glucosinolates contents and profiles in Brassica leaves.
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2005年第4期80-84,共5页
Chinese Journal of Oil Crop Sciences
基金
国家科技攻关(2001BA501A16)
科技部创新基金(03C26214200195)
湖北省科技攻关(2004AA203B01)
关键词
油菜叶片
硫甙
测定方法
Brassica leaves
Glucosinolate
Analysis method
作者简介
李培武（1961-），男，研究员，博士生导师，华中农业大学在读博士，从事农产品质量标准与检测技术研究，中国农科院二级杰出人才，国务院特贴专家，国内外已发表论文82篇，获国内外成果奖励8项。电话：027-86812943，传真：027-86812862．Email：peiwuli@oilcrops．cn。
