摘要
目的:调查血液培养中病原菌的菌群分布及耐药趋势.方法:1995/2004血液培养标本用荧光全自动血液培养仪(Bactec9120)进行培养,阳性标本用全自动微生物鉴定仪(V itek-32)进行鉴定,药敏采用K-B法.结果:在6207份血液培养标本中分离出病原菌662株,阳性检出率为10.7%.病原菌以革兰阳性(G+)需氧球菌居首位(50.3%),革兰阴性(G-)需氧杆菌次之(44.7%),真菌3.0%,厌氧菌1.1%.血液培养中的G+球菌对万古霉素和亚胺培南较为敏感,G-杆菌对亚胺培南、舒普深、丁胺卡那较为敏感.结论:血液培养病原菌以G+球菌为主,G-杆菌次之;儿童血液培养病原菌以葡萄球菌属为主;亚胺培南对G+球菌和G-杆菌均具有较高的敏感率.
AIM： To investigate the distribution and antimicrobial resistance of pathogens isolated from blood specimens in patients with fever in our hospital from 1995 to 2004. METHODS： Blood specimens were cultured by Bactec 9120 Fluorescent Instruments, The colonies of the specimens yielding growth were identified by the Vitek-32 Instruments and K-B method was used for the drug sensitivity tests. RESULTS. Six hundred and sixty-two bacteria strains were isolated from a total of 6207 blood culture specimens, with a positive rate of 10.7%, Of the identified bacteria, G^＋ cocci accounted for 50. 3%, G^- bacilli accounted for 44, 7%, fungi accounted for 3, 0%, and anaerobian accounted for 1.1%. The susceptibility rates of G^- bacilli to Imipenem, Amikacin and cefoperazone/sulbactam were high, The susceptibility rate of G^＋ cocci to vancomycin and Imipenem were also high. CONCLUSION： G^＋cocci prevail over G^- bacilli in the blood specimens, The staphylococci are the chief bacteria in the children＇s blood specimens, Both G^＋ cocci and G^- bacilli are sensitive to Imipenem.
出处
《第四军医大学学报》
北大核心
2005年第23期2133-2135,共3页
Journal of the Fourth Military Medical University
关键词
血液培养
病原菌
抗药性
多药
分离鉴定
blood culture
pathogens
drug resistance,multiple
isolated identification
作者简介
魏军（1961-），女（汉族），甘肃省榆中县人．双硕士学位，主任检验师．Tel．（0951）6744340 E-mail．Lydiajunwei@hotmail．com
