摘要
对分离自我国11个省24个地区49株蚕豆根瘤菌及11株参比菌株进行了唯一碳源、氮源、抗生素、耐逆性和酶活性等138个表型性状测定,并用M INTS软件进行聚类分析。结果表明,全部供试菌株在59%的相似水平上聚在一起,在80%的相似水平上可分为6个群。其中群4与参比菌株聚在一起,而其他5个群均由未知菌组成。进一步对36株菌进行了16S rDNA PCR-RFLP分析,在85%相似水平上供试菌可分为4个群和1个独立的分支,其聚群结果与数值分类结果有较好的一致性。表型及遗传型分析结果表明,我国蚕豆根瘤菌具有极大的多样性。
49 rhizobia strains, isolated from root nodules of broadbean （ Vicaiafaba L. ） in 11 provinces of China were studied by analysis of 138 phenotypic characteristics. All the strains collected together at the boundary of 59% similarity. At the similarity of 80% there were 6 groups which were all composed of strains isolated from the root nodules of broadbean except the cluster 4 included one reference strain. Based on the results of numerical taxonomy, 25 strains as the representation of the broadbean rhizobia with 11 reference strains were selected to PCR-RFLP of 16S rDNA analysis. The strains were clustered into 4 groups at the similarity of 85% with another 1 unattached embranchment. The cluster status of 16S rDNA PCR-RFLP was in good agreement with that of numerical taxonomy on the similarity level of 80%. Overall the results demonstrated a high phenotypic and phylogenetic diversity of rhizobia strains nodulating broadbean.
出处
《微生物学通报》
CAS
CSCD
北大核心
2005年第6期26-31,共6页
Microbiology China
基金
国家973项目资助(No.001CB1089)
关键词
蚕豆根瘤菌
多样性
表型及遗传型特征
Rhizobia sp. Viciafaba L. , Diversity, Phenotypic and phylogenetic characteristics
作者简介
通讯作者,Tel：010-68975891，E-mail：jli@caas.ac．cn.
