摘要
本实验分离培养SD大鼠前体脂肪细胞,以油红O染色计数法区分分化的不同阶段,采用半定量RT-PCR法检测脂肪细胞分化过程中转录因子固醇调控元件结合蛋白(sterol regulatory element binding protein,SREBP-1c)、碳水化合物反应元件结合蛋白(carbohydrate responsive element binding protein,ChREBP)以及脂肪酸合成酶(fatty acid synthase,FAS)、乙酰辅酶A羧化酶(acetyl-CoA carboxylase,ACC1)、硬酯酰辅酶A去饱和酶(stearoyl-CoA desaturase,SCD)和激素敏感脂酶(hormone sensitive lipase,HSL)基因mRNA表达水平的变化。结果表明,上述基因在前体脂肪细胞阶段均不表达,SREBP-1c、FAS在分化初期表达,SREBP-1c、ChREBP、HSL、FAS、SCD在分化中期表达,6种基因在终末分化阶段均有表达。
Primary rat preadipocytes isolated from adipose tissue samples obtained from 20d-old SD rat, respectively, to investigate transcription expression of six genes in adipose tissue in vitro. The amount of triglyceride accumulated was measured through Oil Red O staining was determined to the differentiation stage of adipocytes. The variance was assessed in transcription level of both transcription factors, sterol regulatory element binding protein （SREBP）-1c and carbohydrate responsive element binding protein （ChREBP）, central enzyme genes for fatty acid biosynthesis, acetyl-CoA carboxylase （ACC1） , fatty acid synthase （FAS） and stearoyl-CoA desaturase（SCD）, and lipolytic enzyme, hormone sensitive lipase（HSL） using Semiquantitative RT-PCR analysis. The results had shown .that none genes expression in preadipocyte, SREBP-1c,FAS expression in early stage of differetiation in adipocytes, SREBP-1c,ChREBP,SCD,FAS and HSL expression in midle stage of differetiation in adipocytes, all genes expression in mature adipocytes.
出处
《生物技术通报》
CAS
CSCD
2005年第6期73-76,共4页
Biotechnology Bulletin
基金
国家重点基础研究发展计划(973)专项(NO:2004CB117506)
关键词
脂肪细胞
分化
转录表达
Adipocyte Differetiation Transcription expression
作者简介
陈粉粉（1976-），女，汉族，在读硕士，研究方向：生物技术与动物育种。Tel：029—87091017;E—mail：ffchen03@sina.com
通讯作者：杨公社（1959-），男，汉族，教授，博士，研究方向：生物技术与动物育种。Tel：029—87092430;E—mail：gsyang999@sohu.com
