摘要
背景:应用抖笼换能器法、阈上、阈下剂量戊巴比妥钠睡眠时间的实验法,探讨橙皮水提取物对昆明种小鼠睡眠和自发活动的影响。目的:观察橙皮水提取物对昆明种小鼠睡眠和自发活动的影响。设计:随机对照实验观察。单位:赣南医学院病理教研室、生理学教研室及药理教研室。材料:实验于2005-01/02在赣南医学院科研中心实验室完成。选择健康成年昆明种清洁级小鼠90只,每种实验方法30只,随机分为3组,对照组,小剂量给药组,大剂量给药组;每组10只。方法:①采用抖笼换能器法记录小鼠自发活动情况,小波(波幅<5mm)为小鼠轻度活动(舐毛、瘙痒)的波形;中波(波幅5～10mm)为小鼠中度活动(走动、立起、爬上、爬下)的波形;大波(波幅>10mm以上)为小鼠强度活动(跑动、上串下跳)的波形。小剂量给药组、大剂量给药组分别腹腔注射橙皮水提取物5mg/g、10mg/g和对照组注射等量生理盐水,15min后放回吊笼中,稳定3min后记录15s内小鼠活动波形。②橙皮水提取物对小鼠阈上或阈下剂量戊巴比妥钠睡眠时间的实验法:小剂量给药组、大剂量给药组分别腹腔注射橙皮水提取物5mg/g、10mg/g,对照组注射等量生理盐水;15min后,3组均腹腔注射阈上剂量的戊巴比妥钠(2.5g/L)0.02mL/g,或阈下剂量的戊巴比妥钠(2.5g/L)0.01mL/g,比较给药组与对照组动物的入睡个数、入睡时间和睡眠持续时间。主要观察指标:①小鼠自发活动次数。②阈上剂量戊巴比妥钠小鼠睡眠时间。③阈下剂量戊巴比妥钠小鼠睡眠时间。结果:各实验组小鼠无脱失情况,全部进入结果分析。①橙皮水提取物小剂量给药组,大剂量给药组使中波、大波出现次数显著减少,对小鼠自发活动具有明显的抑制作用,[(90.5±14.7),(58.3±12.5),(45.6±10.1),t=2.341～3.215;P<0.05或P<0.01]。②橙皮水提取物小剂量给药组,大剂量给药组能显著加速阈上剂量戊巴比妥钠的入睡时间和延长戊巴比妥钠的睡眠时间,差异有显著性意义。③橙皮水提取物小剂量给药组,大剂量给药组能显著加速阈下剂量戊巴比妥钠的入睡时间和延长戊巴比妥钠的睡眠时间,差异有非常显著性意义。结论:橙皮水提取物具有明显的延长昆明种小鼠睡眠时间和抑制小鼠自发性活动。与戊巴比妥钠有协同的中枢抑制作用。
BACKGROUND： Effects of Pericarpium aurantiin water extract on sleeping and spontaneous activity in Kunming mice has been investigated in three experiments, that is, the experiments of sleeping time due to supraliminal and subliminal dosage of sodium pentobarbital, and shaking cage for energy transducer, respectively.OBJECTIVE： To observe the influence of Pericarpium aurantiin water extract on sleeping and spontaneous activity of Kunming mice.DESIGN：Randomized controlled trial.SETTING： The Staff Rooms of Pathology, Physiology and Pharmacology in Gannan Medical College.MATERIALS： The experiment was conducted in the Central Laboratory of Science Research, Gannan Medical College from January through February 2005. Ninety adult Kunming mice, grade of clearness, were enrolled and randomly divided into three groups with 30 mice for each experiment：10 in control group, 10 in large-dosage group and 10 in small-dosage group.METHODS： ① In energy transforming trial, spontaneous activity of mice was recorded, small wave with wave amplitude ＜ 5 mm was considered as the waveform of gentle activity in mice, such as fur licking and tickling; middle wave with wave amplitude of 5-10 mm as the waveform of moderate activity, such as walking, standing, climbing up and down;large wave with wave amplitude ＞ 10 mm as the waveform of intensive activity, such as running, jumping up and down. Mice in the small-dosage group, large-dosage group and control group were respectively given intraperitoneal injection of 5 mg/g, 10 mg/g Pericarpium aurantiin water extract and equal volume of normal saline, and placed into the cage after 15 minutes. Then, 3 minutes later, waveforms of mice within 15 s was recorded. ② In the experiments on duration of sleep in mice due to supraliminal or subliminal dosage sodium pentobarbital, 0.02 mL/g or 0.01 mL/g sodium pentobarbital （2.5 g/L） were administrated intraperitoneally into mice in the small-dosage, large-dosage and control group 15 minutes after intraperitoneal injection of 5 mg/g, 10 mg/g Pericarpium aurantiin water extract and equal volume of normal saline were given to the three groups, respectively. Subsequently, comparisons of the number of sleeping mice, time to falling asleep, and sleeping time were carried out among the three groups.MAIN OUTCOME MEASURFS： ① Times of spontaneous activity, ②Sleeping time due to supraliminal dosage of sodium pentobarbital, ③ Duration of sleep due to subliminal dosage of sodium pentobarbital.RESULTS： All the mice were involved in the result analysis without loss.① There were fewer middle and large waves in the small-dosage and largedosage groups as compared with that in the control group （90.5±14.7,58.3±12.5, 45.6±10.1, t=2.341-3.215; P ＜0.05 or P ＜0.01）, indicating that Pericarpium aurantiin water extract can evidently inhibit spontaneous activities in mice. ② In comparison with the control group, the time to falling asleep and the duration of sleep due to supraliminal dosage of sodium pentobarbital were shortened and prolonged significantly, respectively,in the small-dosage and large-dosage groups. ③ In comparison with the control group, the time to falling asleep and duration of sleep due to subliminal dosage of sodium pentobarbital were shortened and prolonged significantly, respectively, in the small-dosage and large-dosage groups.CONCLUSION： Pericarpium aurantiin water extract can prolong the duration of sleep and inhibit the spontaneous activities of Kunming mice,which is in cooperation with sodium pentobarbital in central inhibition.
出处
《中国临床康复》
CSCD
北大核心
2005年第44期173-175,共3页
Chinese Journal of Clinical Rehabilitation
作者简介
缪作华,男,1966生,江西省赣州市人,汉族,1988年江西医学院毕业,学士,讲师,主要从事病理诊断与科研.
曾靖,男,教授,赣南医学院药理教研室,江西省赣州市 341000 通讯作者
