摘要
在粒细胞／巨噬细胞集落刺激因子（ＧＭ－ＣＳＦ）存在下，离体培养人骨髓单个核细胞３周，结果显示有大量具破骨细胞特性的多核细胞形成，细胞核３～２０余个不等，多数细胞显示抗酒石酸盐酸性磷酸酶强阳性反应，当与牙本质片共育３周，可在牙本质片上形成典型吸收陷窝，并可见具有破骨细胞形态样的细胞，提示此培养条件下有大量破骨细胞形成．
Theauthorsdevelopedaculturesystemforhumanosteoclasts.Itwasbasedontheincu-bationofmononuclearbonemarowcelsforthreeweeksinthepresenceofGM-CSF.Theresultingcelsexhibitedaltheclassiccharacteristicsofosteoclasts.Theyformedatruesyncytiumwithmorethan20nucleipercel.Thecelsandpartofthemononuclearcelsrevealedastrongpositivereactiontotartrate-resistantacidphosphatase.Whenthesecelswereculturedonhumandentineslicesforthreeweeks,typicalresorptionpitswereformed.Theauthorsbelievethatthereisthepossibilitytoculturelargenumberofosteoclastunderthisculturecondition.
出处
《中华骨科杂志》
CAS
CSCD
北大核心
1996年第7期465-466,共2页
Chinese Journal of Orthopaedics
关键词
破骨细胞
骨髓培养
Granulocyte-macrophagecolony-stimulatingfactor(GM-CSF)OsteoclastCulture
