摘要
从节旋藻属5个品系和螺旋藻属1个品系中克隆了hoxY基因的部分序列.序列长度都是479bp.在节旋藻中该基因GC含量为46.0%～46.6%,螺旋藻中为43.5%.节旋藻各品系间序列的相似性介于93.7%～100%,明显高于节旋藻属和螺旋藻属间的序列相似性(69.5%～72.2%).2个属中镍铁氢化酶小亚基HoxY氨基酸序列的比较也表明螺旋藻和节旋藻之间存在较大差异.利用MEGA2通过比较核酸序列构建了系统树,表明螺旋藻和节旋藻属处于不同的分枝.
The partial hoxY gene was cloned and characterized from 2 cyanobacterial genera, including 5 Arthrospira strains and 1 Spirulina strain. The gene encodes the small subunit of nickel-iron hydrogenase with the same length of 479 bp in two genera. The GC content ranges from 46.0 % to 46.6 % in Arthrospira and is 43.5 % in Spirulina The similarities of nucleotide sequences among the 5 strains in Arthrospira （93.7%-100%） are higher than those between Arthrospira and Spirulina （69.5%-72.2%）. The alignment of deduced amino acid sequence also indicates that S. subsalsa FACHB351 is phylogenetically distinct from the Arthrospira strains. The phylogenetic tree was constructed by comparing with the corresponding sequences of other 6 cyanobacterial strains. All clade, which is highly supported by the bootstrap values, the 5 strains of Arthrospira form a monophyletic while the Spirulina stain is in another clade.
出处
《中国海洋大学学报（自然科学版）》
CAS
CSCD
北大核心
2005年第6期1021-1025,共5页
Periodical of Ocean University of China
基金
中日据点大学交流合作项目资助
作者简介
张晓辉（1977-）,女,博士,讲师. E-mail：bio-zxh@yahoo.com
张学成,通讯作者：E-mail：xczhang@ouc.edu.cn
