摘要
采用ＬｓＡＢ免疫组比方法，检测了连续２３例新鲜的食管鳞癌手术标本中癌组织Ｐ５３蛋白、增殖细胞核抗原（ＰＣＮＡ）和正常食管粘膜上皮Ｐ５３蛋白的表达。全组病例中癌组织Ｐ５３蛋白表达的阳性率为７３．９％，过度表达为４７．８％，同一病人配对的正常食管粘膜上皮Ｐ５３蛋白表达阳性率７８．３％，过度表达为４．３％。癌组织和正常粘膜上皮Ｐ５３蛋白表达的强度高峰位置呈右移现象，前者的强度高峰在位置上，后者则在±位置上。癌组织中ＰＣＮＡ呈１００％的表达，过度表达为５２．２％。食管鳞癌Ｐ５３蛋白过度表达与肿瘤分级有统计学上的显著性意义，但与肿瘤分期无统计学显著性意义，ＰＣＮＡ的过度表达与肿瘤分级、分期没有统计学上的显著性意义（Ｐ＞０．０５）．本研究提示，食管鳞癌之Ｐ５３蛋白表达高于其他肿瘤，其临床意义和应用价值可能更大，Ｐ５３蛋白和ＰＣＮＡ的表达没有相关关系（ｒｓ＝０．１２３２，Ｐ＞０．ｏ５）。
Expression of P53 protein and proliferating cell nuclear antigen(PCNA)in consecutive 23 surgical samples of squamous cell carcinoma of esophagus and normal esophageal epithelium was studied with immunohistochemical LSAB method from March 1994 to January 1995.73.9% positive and 47.8% overexpression of P53 protein were shown in cancer tissue,and 78.3% and 4.3% respectively for normal esophageal epithelium.A right shift of P53 protein expressive peak was observed from±normal tissue to cancer tissue. PCNA showed 100% positive and 52.2% overexpression. No statistic significance was found among P53 protein,PCNA expression and clinical stage(P> 0.05).There is statistic significance incorrelation between P53 protein and pathological grade(P=0.0495).The study showed that P53 protein expression in squamous cell carcinoma of esophagus was higher than that in other cancers,and the clinical value might be much important.There was no correlation of P53 protein with PCNA(rs=0.1232,P>0.05),so they were independent index in diagnosis,treatment and prognostic study for esophageal cancer.
出处
《肿瘤》
CAS
CSCD
北大核心
1996年第1期11-13,共3页
Tumor
