摘要
目的:观察十二烷基硫酸钠(sodiumdoecylsulfate,SDS)促进血链菌生物膜表面脱落的效果,并对其和氯己定、玉洁纯联合应用的效果进行比较。方法:在模拟人口腔环境的人工口腔内,形成4h血链菌生物膜,分别放入300mmol·L-1SDS、300mmol·L-1SDS+2%氯己定和300mmol·L-1SDS+0.3%玉洁纯中,作用时间为3、10、30min,然后应用激光共聚焦扫描显微镜和死菌/活菌荧光染色技术相结合,比较其促进血链菌生物膜表面脱落的作用,对所得数据进行方差分析。结果:300mmol·L-1SDS应用于4h血链菌生物膜,可以有效促进细菌脱落,300mmol·L-1SDS和2%氯己定联合应用的效果较差;300mmol·L-1SDS和0.3%玉洁纯联合应用于生物膜3min,细菌几乎全部脱落。结论:300mmol·L-1SDS和0.3%玉洁纯联合应用,对促进菌斑生物膜脱落的作用最强。
PURPOSE： To investigate the effects of sodium doecyl sulfate on Streptococcus sanguis biofilm. METHODS： Streptococcus sanguis biofilm was formed on saliva-coated glass（SCG） in a flow culture system, then exposed to different dental plaque control agents for 3,10,30 minutes including A：300mmol·L^-1 sodium doecyl sulfate （SDS）; B： 2% chlorhexidine combined with 300 mmol·L^-1 SDS; C：0.3%triclosan combined with 300 mmol·L^-1 SDS. Confocal laser scanning microscopy and vital/dead fluorescent staining technique（vital stained green, dead stained red） were combined to observe biofilm thickness, bacterial density and viability, analysis of variance was used for comparison. RESULTS： The biofilm thickness and bacteria density reduced after treatment by 300 mmol·L^-1 SDS. However, there was no significant difference after treatment by 2% chlorhexidine combined with 300 mmol·L^-1 SDS; When treated by 0.3% triclosan combined with 300 mmol·L^-1 SDS, most or all of the bacteria detached from the surface and dispersed. CONCLUSIONS： The combination of SDS （aimed at detachment） and triclosan（aimed at killing bacteria） may be most effective in controlling biofilm.
出处
《上海口腔医学》
CAS
CSCD
2005年第5期507-510,共4页
Shanghai Journal of Stomatology
基金
上海市口腔医学重点实验室基金(036505013)~~
作者简介
马瑞（1973-）,女,博士,主治医师
：刘正,Tel：021-63135412,E-mail：marui6868@yahoo.com,通讯作者
