摘要
用淋巴细胞杂交瘤技术,研制出的抗猪细小病毒单克隆抗体,采用酶联免疫吸附试验(ELISA)方法,对本地区所采得的308份猪血清进行检测,阳性检出率达66.6%。其中20～35日龄仔猪阳性检出率在80%以上;45日龄仔猪阳性检出率为75.5%;屠宰猪阳性检出率55.2%。选择70份被检血清用已建立的检测猪细小病毒抗体的 ELISA 试验与传统的 HI 试验进行比较,其敏感性较高,ELISA 检出阳性率为85.7%,HI 检出阳性率为80%,前者较 HI 试验高出5.7个百分点。
308 blood serums from native pigs were detected by ELISA using mono- clonal antibody of anti-ppv produced with lymphoid cell hybridomas technique and the posi- tive detectable rate is 66.6%。Among which the positve detectable rates for 20-35 day-old piglets,45day-old piglets and slaughter pigs are above 80%,75.5% and 55.2% respective- ly.We compared ELISA and traditional HI both are for detecting ppv antibody using selected 70 detected blood serums and the sensibility of ELISA is comparatively higher.The de- tectable positive rates of ELISA and HI are 85.7% and 80% respectively and the former is 5. 7% higher than HI.
出处
《宁夏农林科技》
1996年第1期31-33,共3页
Journal of Ningxia Agriculture and Forestry Science and Technology
