摘要
对国槐等8种(亚种)蝶形花亚科植物组织培养再生能力的研究结果表明:8种槐树叶片的再生能力相差较大,不定芽分化率由高到低排序为金叶槐(79·03%)、黄金槐(41·46%)、香花槐(36·2%)、刺槐(34·2%)、黄花槐(25%)、国槐(22·7%)、四倍体刺槐(21%)、红花槐(18%);愈伤组织诱导率、芽分化率及试管苗的生根率排列次序与叶片芽分化率基本相同。从本试验结果可得出8种槐树基因型对组织培养再生能力具有如下调控效应及特点:属间、种间及亚种间基因型对培养基种类及植物生长调节剂的要求,对脱分化与再分化能力的调控,对不同组织、器官的再生能力的调控均具有同一性;不同基因型、甚至同一基因型的不同组织、器官还具有一定的特异性。
The regeneration ability for 8 pagoda were compared with different genotypes. The result tree varieties of Papilionoideae in the plant tissue culture shows that the buds differentiation rate of leaves varied much with a descending order var. japonica Jinye （79. 03% ） , var.japonica golden （41.46%）, Cladrastis sinensis （36.2%）, Robinia psudoacian L. （ 34.2% ）, Cassia suffruticasa （25%）, Sophora japonica L. （22.7%）, var. robinia tetraploid （21%） and Robinia hispida L. （ 18% ） ; The descending order of induction rate, differentiation rate of cullas and rooting rate was the alide with the bud differentiation rate of leaves. The regulatory effects and traits of genotype on plant regeneration ability was discussed respectively by this experiment system. There were indications that the demand for basic medium and foreign hormone was similar between different genotypes of of pagoda pagoda trees was identical; the and organs, but there still existed the same genotype. tree; the regulation of genotype regulation of genotype had identity specificities in different tissues and on dedifferentiation and redifferentiation on regeneration ability of plant tissues organs of different genotypes as well as the same genotype.
出处
《园艺学报》
CAS
CSCD
北大核心
2005年第5期844-848,共5页
Acta Horticulturae Sinica
基金
辽宁省大连市科技攻关项目(2001-0745)
关键词
槐树
组织培养
基因型
再生能力
Pagoda tree
Genotype
Tissue culture
Regeneration ability
