摘要
目的:以非小细胞肺癌(non-small cell lung cancer,NSCLC)为对象,探讨肿瘤局部微环境中免疫反应的特点及其对抗肿瘤免疫的影响。方法:以5例结核性胸膜炎患者作为对照,采用地高辛末端标记的寡核苷酸探针,以原位杂交技术检测了23例非小细胞性肺癌(NSCLC)患者的新鲜胸腔积液和/或手术切除标本中的淋巴细胞和肿瘤细胞中,IL-2、INF-γ、IL-12(p40)、IL-18、IL-4、IL-10、TGF-β1、IL-1、IL-3、IL-8、GM-CSF、TNF-α及TGF-αmRNA的表达。结果:NSCLC患者胸腔积液的单个核细胞及肿瘤组织中,IL-4、IL-10、TGF-α和TGF-β1mRNA的表达水平,明显高于IL-2、IL-12、IL-18和INF-γmRNA的表达;而结核性胸腔积液的单个核细胞中上述细胞因子mRNA的水平均降低,并且各细胞因子mRNA的表达水平之间没有明显的差异。结论:NSCLC患者胸腔积液的单个核细胞及肿瘤组织中,II型细胞因子和免疫抑制细胞因子mRNA的表达占主导地位,反映了肿瘤局部的免疫微环境处于抑制状态。上述结果有助于了解肿瘤逃逸的机制,并为制定NSCLC免疫治疗的方案提供了重要的实验依据。
AIM： Using Non-small cell lung cancer （NSCLC） as subject, to explore the characteristics of immune response in immunological microenvironment at the tumor site and its effect on anti-tumor immunity. METHODS： Using in situ hybridization （ISH）, the expressions of IL-2, INF-γ, IL-12 （p40）, IL-18, IL-4, IL-10, TGF-β1, IL-1, IL-3, IL-8, GM-CSF, TNF-α and TGF-α mRNAs in lymphocytes and tumor cells from five fresh pleural effusion sam- ples and 18 tumor tissue samples of NSCLC patients were detected by in situ hybridization with digoxin end-labeled oligonucleotide probe, using 5 tuberculous pleurisy patients as control. RESULTS： In pleural effusion and tumor tissue of NSCLC patients, the expression levels of IL-4, IL-10, TGF- α, and TGF-β1 mRNAs were significantly higher than those of IL-2, IL-12, IL-18 and INF-γ, mRNAs. In contrast, the analysis of tuberculous pleural effusion samples revealed lower levels of above-mentioned cytokine mRNA. There was no significant difference among expression levels of these cytokine mRNAs. CONCLUSION： The expressions of type II cytokine mRNAs and immunosuppressive cytokine mRNAs in pleural effusion and tumor tissue of NSCLC patients occupied a dominant position, suggesting that the immunological microenvironment about tumor be in an immunosuppressive state. The present study has contributed to a better understanding the mechanisms of tumor escape and provides important experimental basis for working out the scheme for an effective immunomodulatory treatment of NSCLC patients.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2005年第6期763-766,共4页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金资助项目(No.30370549)
关键词
非小细胞性肺癌
细胞因子
原位杂交
non-small cell lung cancer
cytokine
in situhybridization
作者简介
陈宏伟（1966-），男，陕西西安人，主治医师，讲师，博士．
Corresponding author，Tel：（029）82655499 Email：wangyili@mail．xjtu．edu．cn.
