摘要
目的探讨金荞麦乙醇提取物乙酸乙酯的萃取部分(待测样品)的抑菌作用,并对此部分进行分离纯化以研究其抗菌活性的物质基础。方法与复方板蓝根颗粒做对比,采用平皿稀释法及动物实验对待测样品进行体外及体内实验,测定其对各试验菌的最小抑菌浓度(M IC)和对肺炎链球菌感染小鼠的体内保护作用。并采用多种色谱方法对此部分进行分离纯化。结果体外抑菌试验表明,待测样品对乙型溶血性链球菌、肺炎球菌有明显的抑制作用;体内抑菌实验表明此部分对肺炎球菌菌株所致的小鼠感染有保护作用;从该活性部分分离得到8个化合物为:反式对羟基桂皮酸甲酯(trans-p-hydroxy c innam ic m ethy l ester,I),3,4-二羟基苯甲酰胺(3,4-d ihydroxybenzam ide,II),原儿茶酸(protocatechu ic ac id,III),原儿茶酸甲酯(protocatechu ic ac id m ethy l ester,IV),木犀草素(lu teo lin,V),槲皮素(querc itrin,V I),芸香苷(ru tin,V II),(-)-表儿茶素[(-)-ep icatech in,V III]。结论待测样品在体内及体外均有较强的抑菌活性,而具有抗菌作用的物质基础为酚酸类及黄酮类化合物。
Objective To study on bacteriostasis of extract of Fagopyrum cymosum （Trey.） Meisn. with ethanol and ethyl acetate. Then separated this partion. Methods Compared with Banlangen, minimal inhibitory concentration （MIC） in vitro and the protection in vivo to the mice which infected with Streptococcus pneumoniae of the extract effecting on bacteria was assayed by petri dish dilution and zoopery. By solvent extraction, various method of isolation. Results This extract had different bacteriostastic activity on different Bacillus. The second of Hemolytic streptococcus and Pneumococcus were obvisously resrained. And 8 compounds were isolated and purified from this partion. Conclusions The extract of Fagopyrum cymosum （Trey.） Meisn. with ethanol and ethyl ace tate had obvisously resrained on the second of Hemolytic streptococcus and Pneumococcus. The compounds isolated from the partion presume bacteriostastic activity .
出处
《中国微生态学杂志》
CAS
CSCD
2005年第5期330-331,共2页
Chinese Journal of Microecology
关键词
金荞麦
抑菌作用
化学成分
抗菌活性
Fagopyrum cymosum
Bacteriostasis
Chemical constituent
作者简介
王立波（1980-）,女,在读硕士,从事天然药化研究;
吴立军,通讯作者,E-mail：wulijun_111@hotmail.com
