摘要
目的:观察实验性哮喘大鼠一氧化氮合酶/一氧化氮系统的改变,以探讨NO的生成机制。方法:用卵蛋白致敏并激发建立了SD大鼠哮喘模型,32只大鼠随机分为4组,每组8只,为正常对照A组、卵蛋白激发哮喘发作B组、L-精氨酸对照C组、L-精氨酸+卵蛋白刺激D组,用铬粒还原法测定血浆中硝酸盐水平,免疫组化观察iNOS的表达情况。结果:正常大鼠各级支气管、肺泡管、肺泡囊上皮细胞均呈iNOS阳性反应,气道上皮下平滑肌呈iNOS阴性反应;哮喘大鼠各级支气管、肺泡管及肺泡囊上皮细胞呈iNOS强阳性反应,气道上皮下平滑肌呈iNOS阳性反应,且浸润的炎细胞也呈强阳性表达;L-精氨酸对照组大鼠各级支气管、肺泡管、肺泡囊上皮细胞均呈iNOS阳性反应,气道上皮下平滑肌呈iNOS弱阳性反应;L-精氨酸+卵蛋白激发组大鼠各级支气管、肺泡管、肺泡囊上皮细胞均呈iNOS阳性反应,气道上皮下平滑肌呈iNOS阳性反应。与A组相比,B组大鼠SOD活性明显下降(P<0.01),MDA水平明显增高(P<0.01),B、D组大鼠NO3-水平明显增高(P<0.05),C组与A组比较没有明显变化。结论:L-精氨酸对哮喘大鼠NOS/NO的产生具有调节作用。
Objective：To investigate the effects of L-arginine on nitric oxide （NO） and the expression of iNOS in the airway inflammation of asthma. Methods：the rat models of asthmatic inflammation were established by sensitizing and then challenging the animals with ovalbumin. The 32 animals were randomly divided into control group, sensitized group, L-Arginine control group as well as sensitized and L-Arg-treated group equally, iNOS impression was observed by using immunohistochemistry method, and The nitric oxide concentration was detected by the activated cadmium reduction method. Results：iNOS expression in athmatic rats was positively increased compared with that of the control group, nitric oxide concentration was obviously increased correlated with the blood gas getting worse. In sensitized and L-Arg-treated group, nitric oxde concentration was also increased while There was no statistically difference in the impression of iNOS and blood gas .Condusions ： It was indicated that a suitable levels of L- arginine can influence the production of nitric oxide while not change the expression of iNOS, L-arginine may inhibit the attack of asthma by promoting intrinsic NO induced by cNOS not iNOS.
出处
《军医进修学院学报》
CAS
北大核心
2005年第5期326-328,共3页
Academic Journal of Pla Postgraduate Medical School
基金
"十五"军队科研基金项目(01MB072)
作者简介
宋淑珍（1970-），女，河北保定人，2002年军医进修学院硕士毕业，主治医师，发表文章11篇。电话：（010）66936214；E—mail：song0602@sohu．com
[通讯作者]田亚平，电话：（010）66939374；E-mail：tianyp@301hospital．com．cn
