摘要
应用AFLP技术初步研究20个梅花株系间的亲缘关系,19对选择性引物扩增出清晰的总位点数918个,其中多态性位点324个,占总位点数的35.29%。采用SAS类平均法聚类分析AFLP数据,λ值为0.9759时,将20个梅花株系分为4个类群。半野生种曲梗梅的多态性位点数最高,‘多萼朱砂’、‘复瓣小宫粉’等梅花品种次之。在类群Ⅳ中,具有明显亲缘关系的两组亲本及其杂交子代各自聚为一组,表明AFLP技术和聚类分析方法可以用来研究梅花品种间的亲缘关系。‘小宫粉’、‘江南朱砂’和它们的子代亲缘关系密切,结果仍能区别其较小差异,这有助于解决梅花中存在的不同产地相同品种异名和同名而品种不同的问题。垂枝类梅花品种间有较近的亲缘关系或者遗传来源,支持枝姿作为梅花分类的重要形态特征,垂枝类品种属于梅花中比较进化的类群与其历史演化相吻合。本研究中所采用的DNA限制性内切酶酶切组合及所筛选的AFLP选择性引物对适合研究梅花品种间的亲缘关系和遗传多样性。
The genetic relationships among a variety, a hybrid and 18 Mei flower cultivars were preliminarily investigated using AFLP. Nineteen selected primer combinations revealed 918 legible loci, 324 of which were polymorphic ones that accounting for 35.29% of the total. Based on the SAS average similarity cluster analysis, 20 Mei samples were classified into four groups as λ=0.9759. P. mume var. cernua showed the highest polymorphism among these samples. ‘Duoezhusha＇ and ‘Fubanxiaogongfen＇ showed less polymorphism than P. mume var. cernua. In the group Ⅳ, AFLP and cluster analysis results were as follows： Parentages of some Mei flower cultivars were successfully identified. The results showed differences between ‘Xiaogongfen＇, ‘Jiangnanzhusha＇ and their hybrids which would be useful to clear the confusions of homonyms and synonyms. There were close genetic relationships among cultivars of pendulous group, which supported traditionally classified system using branch gesture as the important morphological character in Mei flowers. The pendulous cultivars were more evolutionary than others, which is in conform with their cultivated history. These results demonstrate that AFLP technique is an efficient method for evaluating the genetic relationships and diversity of Mei flower.
出处
《中国农业科学》
CAS
CSCD
北大核心
2005年第10期2084-2089,共6页
Scientia Agricultura Sinica
基金
国家自然科学基金资助(30170666)
作者简介
杨朝东（1971-），男，博士，主要从事园林植物遗传改良和生物技术研究。
包满珠为通讯作者，Tel：027-87282435；Fax：027-87282095；E-mail：mzbao@mail．hzau．edu．cn
