摘要
从感病的柞蚕Antheraeapernyi蛹中分离纯化柞蚕核型多角体病毒(ApNPV),提取基因组DNA,分别构建ApNPVDNA的HindⅢ和SalⅠ酶切片段文库。对基因文库中1个克隆进行序列分析,得到1个长度为321bp的序列,其中包含一个编码76个氨基酸的开放阅读框,预测的分子量为8.46kD,系泛素类似基因。在读码框的上游调控序列中,具有典型的晚期基因启动子序列ataag。氨基酸序列同源性分析结果表明,ApNPV与黄杉毒蛾Orgyiapseudotsugata核型多角体病毒(OpNPV)的同源性最高(96.1%),与苜蓿尺蠖Autographacalifornica核型多角体病毒(AcNPV)的同源性为86.8%,与棉褐带卷蛾Adoxophyesorana颗粒体病毒(AoGV)的同源性最低(71.1%),但与人类、线虫和酵母的泛素同源性分别为77.6%、76.3%和76.3%。一些氨基酸残基在真核生物中保守,在杆状病毒中不保守,个别氨基酸残基是杆状病毒所特有的,这些氨基酸序列的改变对杆状病毒泛素基因的作用有待进一步研究。
Polyhedron particles of Antheraea pernyi nuclear polyhedrosis virus （ApNPV） were purified from A. pernyi pupa. The genomic DNA of ApNPV was extracted, and the viral DNA was digested by Hind Ⅲ and Sal Ⅰ , respectively. A viral fragment with a length 321 base pairs was sequenced, which contains a 76 amino acid residues open reading frame with deductive a molecular weight 8.46 kD, homologous to ubiquitin. By comparing the identity of amino acid sequence of the gene with that of baculoviruses and other organisms, it was found that ApNPV ubiquitin gene had the highest identity （96.1% ） with Orgyia pseudotsugata multinucleocapsid nuclear polyhedrosis virus （OpNPV）, the second high identity with Autographa californica NPV （AcNPV, 86.8% ）, and the lowest identity with Adoxophyes orana granulovirus （AoGV, 71.1% ）, while had 77.6 % to 76.3 % identity with that of human, nematode and yeast. Although a number of amino acid residues are conservative in the eukaryotic ubiquitin, they are diverse in baculovirus ubiquitin gene. Several amino acid residues are unique to the baculovirus ubiquitin genes.
出处
《昆虫学报》
CAS
CSCD
北大核心
2005年第5期819-822,共4页
Acta Entomologica Sinica
基金
江苏省教育厅基金项目(02KJD180003)
江苏大学高级人才基金(1683000023)
关键词
柞蚕
核型多角体病毒
泛素
序列分析
Antheraea pernyi
nucleopolyhedrovirus
ubiquitin
sequence analysis
作者简介
王利群，女，1972年生，江苏常州人，博士，研究方向为昆虫病毒分子生物学，E-mail：h_567@sina．com
通讯作者，E-mail：wenbingwang@ujs．edu．cn
