摘要
葫芦素B是从甜瓜茎中提取的药用成分,以前的研究成果已经发现,该药具有保肝作用。但是其作用机制还不是很清晰,本实验探讨的是葫芦素B是否通过抑制凋亡信号转导而达到保肝的目的。过氧化氢诱导的乳鼠凋亡模型,然后给与不同剂量三个浓度的葫芦素B(3、10、30mmol/L)。通过流式细胞计量术测量细胞早期凋亡率。与模型组比较,高中剂量组能明显降低肝细胞早期的凋亡率(p<0.05)。上述结果表明葫芦素B能抑制过氧化氢诱导的乳鼠肝细胞的凋亡。
Cucurbitacin B is a pharmaceutical component extracted from stalk of muskmelon. Previous studies have demonstrated that it can prevent damage of hepatocytes. But this mechanism was not cleared yet. The aim of our work is to study cucurbitacin B protecting effects on hepatocytes associated with inhibiting apoptosis signals transduction. The apoptosis model of neonate rat was constructed by induction of H2O2, and then hepatocytes were treated with cucurbitacin B in three concenwations of various dose（3,10,30mmol/L respectively）.Flow cytometry were conducted to investigate early apoptosis ratio. The ratio of early apoptosis cells in high（21.4%） and middle dose groups（36.2%） was decreased significantly compared with model group（68. 3%, p〈0.01 ）. The results suggested that cucurbitacin B could inhibit the apoptosis of neonate rat hepatocytes induced by H2O2.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2005年第9期527-530,共4页
Food Science
基金
长春市科技局新星创业计划项目(长科技合(2004283)号)
关键词
葫芦素B
凋亡
肝细胞
cucurbitacin B
apoptosis
hepatocytes
作者简介
曹淑桂，58岁，女，教授，博士生导师，研究方向为酶与酶工程。
通讯作者
