摘要
对FDA-PI双色荧光法检测水华鱼腥藻和铜绿微囊藻的活性进行了研究,用荧光显微镜对染色结果进行测定.结果表明,在蓝色光激发下(495nm),活细胞被双醋酸荧光素FDA染成亮绿色,死亡细胞被碘化丙锭PI染成红色.染色效率与原植体类型和细胞密度有关.对细胞密度为6×107—7×109个·l-1的铜绿微囊藻,FDA染色效率可达94%以上.对细胞密度为4×107—5×108个·l-1的水华鱼腥藻,FDA染色效率可达91%以上,但细胞密度增大到5×109个·l-1时,由于藻丝体易卷曲在一起,FDA染色率下降到67%.对死亡细胞,PI染色率基本都可达到100%.因此,用FDA-PI检测活细胞和死亡细胞混合的细胞悬液,可根据细胞所发出的不同荧光而判断细胞活性.
The paper studied the viability determination of two kinds of algae Anabaena flos-aquae and Microcystis aeruginosa by FDA-PI double staining. Fluorescence emission from cells was measured by fluorescence microscopy. The results indicated that viable and dead cells were stained as bright green and red fluorescent cells respectively by fluorescein diacetate and propidium iodide under blue excitation （495nm）. The staining rate has relation with the cell density. If the cell density of Anabaena flos-aquae and Microcystis aeruginosa was 6 × 10^7--7 × 10^9cells · l^-1and 4 × 10^7--5 × 10^8 cells · l^-1 , the FDA staining rate would be more than 94% and 91%. But if the cell density of Anabaenaflos-aquae increased to 5 × 10^9 cells · l^-1, the staining rate would fall to 67% obviously due to cell twisting together. The staining rate of PI can reach 100% for the two kinds of algae. Based on the fluorescent light from cells, it can be easy to determinate the cell viability. FDAPI double staining method is a new method to measure the algae cell viability.
出处
《环境化学》
CAS
CSCD
北大核心
2005年第5期554-557,共4页
Environmental Chemistry
基金
国家自然科学基金(50478115)
国家自然科学基金重点基金(20337020).
作者简介
联系人 E-mail：jhqu@mail.rcees．ac.cn
