摘要
本文采用差速离心法分离提纯葱病毒并对其特性进行研究．病毒粒子为弯线性，平均长度７２８ｕｍ，直径约１５ｎｍ，紫外吸收曲线最高峰在２５６～２６０ｎｍ之间，最低峰为２３８ｎｍ，分子量为２７０００道尔顿。光学显微镜下可观察到病叶表皮细胞中的不规则内含物，超薄切片电镜观察，可见病毒风轮状、束状内含体。该病毒引起叶绿体、核膜损伤和解体，其寄主范围较窄。
The results obtained from electron microscopic. observation show that Welsh-onion yellow stripe virus (WoYSV) particle is approximately 728um in length, andmost of them are between 600um and 700um. The purfied virus has a typical UV absorption spectrum max. at 256-260um and min. at 238um respectively. Its average molecularweight is about 27000 Dalion. Pinwheel-like inclusion bodies and destroyed chloroplasts have been found in the infected-leaf cells. Under microscope a number of contentsare observed in the infected cells, including round and elipse ones. They have looserstructure compared with nucleus. Some contents nearby the nucleus form comet-likecomplex bodies with nucleus. The host range of WoYSV is narrow.
出处
《山东农业大学学报（自然科学版）》
CSCD
1996年第3期303-310,共8页
Journal of Shandong Agricultural University：Natural Science Edition
基金
山东省自然科学基金
关键词
葱
病毒
超微结构
Allium fitulosum L.
Welsh-onion yellow stripe virus
Ultrathin sections
