摘要
目的:探讨重型急性胰腺炎(Severeacutepancreatitis,SAP)免疫功能的变化与细菌移位的关系。方法:将Wistar大鼠20只随机分成两组:SAP模型组(M组,n=10),对照组(C组,n=10)。以牛磺胆酸钠胰胆管内注射诱发大鼠SAP模型,流式细胞仪检测大鼠外周血T淋巴细胞亚群CD4+、CD8+细胞百分比并计算CD4+/CD8+的值;对血液、胰腺和肠系膜淋巴结进行细菌培养。结果:①重型胰腺炎血中CD4+细胞数以及CD4+/CD8+值明显低于对照组(P<0.01)。M组细菌培养阳性率明显高于对照组(P<0.01)。结论:①胰腺炎时存在细胞免疫功能紊乱。②肠细菌移位与机体的细胞免疫功能紊乱有关。
Objective： To investigate the relationship between the changes of immune function and the bacterial translocation in experimental severe acute pancreatitis（SAP） in rats. Methods： Twenty Wistar rats were randomly divided into two groups ： models group of SAP （M, n=10） ;control group （C, n=10）. SAP group were induced by injecting adversely 5 % sodium taurocholate into the common biliary-pancreatic duct. The per-centages of CD4^＋ and CD8^＋ lymphocyte were determined by theflow cytometry. Bacterial culture in pancreas,blood and mensentericlymph nodes（MLN） were made. Results： Thepercentages of CD4^＋ and CD4^＋/CD8^＋ratio in SAP group were significantly lower than those in control group（P〈0.01 ）. However the positive ratesofcultured bacteria were significantly higher than that of control group （P〈0.01）. Conclusion： （1） There exists damage of cellular immunity function in pancreatitis; （2）There is relationship between intestinal bacterial translocation and danmge of cellular immune function.
出处
《海南医学院学报》
CAS
2005年第4期254-257,共4页
Journal of Hainan Medical University
作者简介
姜良富（1973-），男，山东省临沂市人，山东省临沂市人民医院外科主治医师，硕士。
