摘要
本文研究了溶液ｐＨ值、配体及某些重金属离子对细胞色素ｃ电化学活性的影响．实验发现：在ｐＨ＝２．０和ｐＨ＝１０．０的磷酸盐缓冲溶液中，细胞色素ｃ失活；用强的配体ＣＮ－与铁卟啉发生配位取代反应虽使溶液相中的细胞色素ｃ失活，但不影响吸附态的细胞色素ｃ的活性；重金属离子Ｈｇ２＋、Ｃｄ２＋、Ｐｂ２＋对细胞色素ｃ电化学活性影响的程度不同，影响大小的顺序为Ｈｇ２＋＞Ｃｄ２＋＞Ｐｂ２＋。并对可能的机理进行了讨论。
The effects of pH, ligtlnd CN￣-,metallic ions,Hg￣(+2), Cd￣(+2) and Pb￣(2+) on electroactivity of cytochrome c have been studied with cyclic voltammetry and UV/Vis spectroseopy.In the phosphate buffer solution containing 5 mg/mL cytochrome c, cytochrome c is electroactive at a glod electrode with suitable pretreatment if the pH value is equal to 7.0, but it is electroinactive at the gold electrode if the pH value is equal to 2. 0 or 10.0. The existence of CN- ions in the solution would cause deactivation of cytochrome c in the bulk sulurion, and would not affect the electroactivity of cytochrome c adsorbed on the gold electrode。The sequence for effects of metallic ions on electroactivity of cytochrome c is Hg￣(+2)>Cd￣(+2)>Pb￣(2+).The possible mechanisms were proposed.
出处
《电化学》
CAS
CSCD
1996年第1期61-65,共5页
Journal of Electrochemistry
基金
国家自然科学基金
