摘要
目的探讨乳腺癌白介素-8(IL-8)基因表达与临床病理因素的关系及意义。方法应用实时定量PCR方法检测110例乳腺癌及正常组织中的IL-8 mRNA表达,计算肿瘤(T)及正常组织(N)的比值(T/N),并采用免疫组化法检测乳腺癌组织中雌激素受体(ER)、孕激素受体(PR),分析IL-8基因表达与临床病理因素的关系。结果乳腺癌组织中均可以检测到IL-8 mRNA表达,若以T/N≥2为标准,55.5%(61/110)的乳腺癌中IL-8基因表达增高。IL-8基因表达T/N水平与腋窝淋巴结转移、激素受体状况有关,而与病人年龄无关。肿瘤>5 cm者IL-8基因表达低于肿瘤≤5 cm者,但无统计学差异。结论乳腺癌IL-8基因表达与部分临床病理因有关,能在一定程度上反映病人的预后。
Objective To evaluate the clinical significance of interleukin-8 （IL-8） gene ex- pression in breast cancer. Methods The IL-8 mRNA on breast was measured by a real-time quantitative PCR in 110 cases of breast cancer and adjuvant normal breast tissues. The ratio of tumor to normal tissue （T/N） of IL-8 expression was then calculated for each case. Estogen recepot（ER） and progestgen receptor（PR） were tested by immunohistochemical assay in 110 cases of breast cancer. This value of IL-8 expression was correlated with the clinical-pathological parameters. Results IL- 8 mRNA was detectable in all tumors tested. Using T/N ratlo≥2 as cut-off point, 55.5% （61/ 110） of tumors were defined as high expression. IL-8 expression was not significantly related to the patient. Similarly, the patients with tumor size〉5 had higher IL-8 expression than those other tumor sizes, but there are no significant statistical difference between them. Significant correlation was found between the T/N ratio and lymph node involvement. IL-8 expression was clearly overexpressed in most ER-negative breast cancer, whereast no significant level coulded bedetdcted in breast cancer. Conclusion In breast cancer, the IL-8 gene expression is highly expressed and associated with some clinical factors and pathological features, possibly it is a good prognostic factor.
出处
《实用临床医药杂志》
CAS
2005年第8期5-7,共3页
Journal of Clinical Medicine in Practice
基金
江苏省常州市卫生局基金资助课题(常卫科教(2002)202号)
关键词
乳腺癌
白介素-8
雌激素受体
孕激素受体
breast Cancer
interleukin-8
estrogen teceptor
progesterone receptor
作者简介
方琦（1972-），女，江苏常州人，主治医师。
