摘要
目的:研究野生型p53基因重组腺病毒载体(AdCMV-p53)导入对U937细胞分化、凋亡和清道夫受体CD36表达的影响。方法:AdCMV-p53导入U937细胞后,用细胞计数、细胞周期分析、台盼蓝染色排除法计数细胞悬液中的活细胞数目和NBT还原反应观察其对U937细胞生长、分化的影响;RT-PCR、免疫荧光和流式细胞分析检测AdCMV-p53导入对CD36表达的影响。结果:AdCMV-p53可以高效导入U937细胞,野生型p53基因导入促进U937细胞向巨噬细胞分化,台盼蓝染色发现实验组阳性细胞数(64.6±9.2) %较对照组(14.2±5.5) %明显增多,吞噬能力增强;NBT还原反应实验组(49.7±12.6) %较对照组(6.3±1.8) %升高。RT-PCR和流式细胞分析检测,野生型p53基因导入使得CD36 mRNA转录增强,CD36蛋白表达增加。结论:野生型p53基因能影响细胞分化和凋亡,并上调清道夫受体CD36的表达,对于动脉粥样硬化的预防和基因治疗具有潜在意义。
AIM： To study the effect of wild- type p53 gene on the differentiation, apoptosis and expression of scavenger receptor CD36 in U937 cells. METHODS： Recombinant adenovirus vector with wild - type p53 gene was constructed and used to transfect U937 cells. With the expression of wild - type p53 gene following adenoviral infection, transfected U937 cells were largely promoted to differentiate into macrophages. RESULTS： Trypanblue- staining test demonstrated that the percentage of positive cells increased from （14.2 ±5.5）% to （64.6± 9.2）% and nitroblue tetrazolium （NBT） reduction test reached similar results （6.3±1.8）% vs （49.7 ± 12.6）%. Furthermore, CD36 mRNA was up- regulated as confirmed by RT- PCR. The increased expression level of CD 36 was also detected by flow cytometry analysis. CONCLUSION： These resuits suggest that wild- type p53 gene can affect U937 cells differentiation and apoptosis, up - regulate expression of scavenger receptor CD36. It may have a potential significance on atherogenesis.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2005年第9期1752-1757,共6页
Chinese Journal of Pathophysiology
基金
Supported by grantsfromThe National Foundationfor Natural Sciences of China (No.30200103)
Hunan Province Ed-ucational Office Foundation of China (No.02B039) .
作者简介
Corresponding author Tel： 0086 - 10- 65130302; E- mail： lijliy@hotmail, com
