摘要
120只1日龄固始鸡随机分为2组,分别饮用添加硼0、400 mg/L的水,研究了硼中毒对固始鸡空肠发育的影响.试验期为6周,每周定时每组取鸡10只,颈静脉放血致死,取空肠,Bouin's液固定,制作石蜡切片,HE染色,显微镜观测与拍照.结果:(1)试验组空肠绒毛长度极显著低于同周龄对照组(P＜0.01);肠腺长度第1周龄极显著低于对照组(P＜0.01),第2周龄显著低于对照组(P＜0.05).(2)试验组1～4周龄空肠出现上皮细胞肿胀、脱落,绒毛中轴结缔组织水肿、毛细血管扩张,肠腺囊肿等病变.试验表明,硼中毒对固始鸡小肠绒毛及肠腺发育有明显抑制和毒害作用,对机体消化吸收功能产生严重影响.
A total of 120 Gu-shi chickens,day-old were randomly divided into two groups. The water was supplemented with 0,400 mg/L boron. The effect of boron toxicosis on jejunum development in Gu-shi chickens was studied. Ten chickens in each group were selected and slaughtered at interval time till the sixth week. The jejunum were sampled ,fixed in Bouin＇s liquid, made into paraffin section,stained with HE,observed by microscope,measured by micrometer and photographed by micro-projector. The results were as follows： （1）There was significant difference （P〈0. 01） between length of villus jejunum of experimental group and that of control group. Compared with control group,the length of intestinal gland was obviously low at 1 week （P〈0. 01） and 2 weeks （P〈0. 05）. （2）The tumefaction and come off of villus epithelia of jejunum,dropsy of connective tissue and dilatation of capillary vessel in villus,and cyst of intestinal gland were observed at 1 to 4 weeks in experiment groups. The results indicated that the boron toxicosis could inhibite obviously the jejunum development and thereby disturb its function.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2005年第5期514-517,共4页
Chinese Journal of Veterinary Science
基金
安徽省优秀青年科技基金资助项目
关键词
硼中毒
固始鸡
空肠
发育
boron toxicosis
Gu-shi chickens
jejununl
development
作者简介
王珏（1950-），女，副教授。E-mail：wangjue888888@yahoo．com．cn。
通讯作者。
