摘要
目的:探讨两种免疫抑制分子人类白细胞表面抗原HLA-G和Fas配体(FasL)与经典型肾透明细胞癌(ccRCC)分级和分期的相关性。方法:采用免疫组织化学方法对60例ccRCC标本石蜡切片进行检测,数据由SPSS软件进行统计分析。结果:肿瘤旁正常肾组织中无HLA-G表达;肾癌组织中,40例(66.7%)表达HLA-G,36例(60.0%)表达FasL,31例(51.7%)两者均表达,15例(25.0%)两者均不表达。在同一标本中,肿瘤侵犯脉管或淋巴结后,其HLA-G或FasL表达较原位肿瘤明显增强。统计分析显示HLA-G表达的阳性率和表达强度与肿瘤的分期、分级均呈正相关(P<0.01)。FasL表达的阳性率与肿瘤分级呈正相关(P<0.05),而表达强度与肿瘤分期呈正相关(P<0.01)。结论:ccRCC中HLA-G和FasL的表达与肿瘤的分期、分级均呈正相关,与淋巴结转移有一定关系;晚期ccRCC高表达免疫抑制分子的机制需进一步研究。
Objective：To investigate the expression of two kind of immunosuppressive molecules, nonclassical human leucocyte antigen class HLA-G and Fas ligand （FasL）, in the conventional （clear cell） renal cell carcinoma （ccRCC）. Methods：60 cases of ccRCCs formalin-fixed, parafflin embeded surgical specimens were examined by immunohistochemistry. The data were statistically analysed by SPSS software. Results： There was no expression of HLA-G in the normal renal tissue. HLA-G was detected in 40 ccRCC cases （66.7%） ,FasL was detected in 36 cases （60.0%）. Both the HLA G and FasL were positive in 31 cases （51.7%） and were negative in 15 cases （25.0%）. Both the HLA-G and FasL expression level were increased after the ccRCC cells invaded the vessel or lymphatic node. The incidence and level of HLA-G were correlated with tumor grade or stage（ P〈0.01）. The incidence of FasL expression were correlated with the tumor grade （ P 〈0.05）but the level of FasL expression were correlated with the tumor stage （ P 〈0.01 ）. Conclasions：The expression of HLA-G and FasL were correlated with the tumor stage, grade and metastasis of ccRCC. The mechanisms of high expression of immunosuppressive mole cules in the advanced stage ccRCCs were required further investigation.
出处
《临床泌尿外科杂志》
2005年第9期567-570,573,共5页
Journal of Clinical Urology
基金
安徽省自然科学基金资助项目(No:050430704)
