期刊文献+

化学诱导表达系统及其在植物中的应用 被引量:4

Chemical-inducible Expression Systems and Advances of Their Applications in Plants
在线阅读 下载PDF
导出
摘要 化学诱导启动子可以在特定时间和部位激活或抑制目的基因的表达。目前,已经建立了多种化学诱导表达系统,用于基因功能分析、无标记植物转化、特定位点DNA切除、育性恢复和RNA沉默等方面的研究。化学诱导表达系统为基础分子生物学研究和生物技术应用提供了强有力的工具,将大大加快植物转基因技术的应用。 Chemical-inducible promoters can activate or inactivate target gene expression at a particular developmental stage and for a specific duration. Several systems have been developed and used to analyze gene function, marker-free plant transformation, site specific DNA excision, restoration of male fertility and RNA silencing. Chemical-inducible expression systems will be powerful tools for basic research in molecular biology and biotechnological applications, which will dramatically increase the application of transgenic technology.
出处 《细胞生物学杂志》 CSCD 2005年第4期407-413,共7页 Chinese Journal of Cell Biology
基金 国家高技术研究发展计划(863计划)资助项目(No.2002AA207013)~~
作者简介 通讯作者。Tel:0571-86971722,E-mail:glu@zju.edu.cn
  • 相关文献

参考文献6

二级参考文献74

  • 1王念捷,程奇,任延国,章力建,沈桂芳.水稻4CL基因启动子引导的GUS在紫外诱导下的组织特异性表达[J].中国农业科学,1996,29(1):73-77. 被引量:6
  • 2[1]Gatz C.and Quail P. H., Proc. Natl. Acad. Sci. USA. (1988)85:1394-1397.
  • 3[2]Gatz C.,Kaiser A.and Wendenburg R., Mol. Gen. Genet. (1991)227:229-237.
  • 4[3]Gatz C., Frohberg C. and Wendenburg R., The Plant Journal (1992)2(3) :397-404.
  • 5[4]Roder F.T. ,Schmulling T.and Gatz C., Mol Gen Genet (1994)243:32-38.
  • 6[5]Faiss M.et al. ,The Plant Journal (1996)10:33-46.
  • 7[6]Faiss M.et al., The Plant Journal (1997) 12:401 -415.
  • 8[7]Rieping M.et al. ,Plant cell (1994) 6:1087-1098.
  • 9[8]Masgrau C.et al. , The Plant Journal (1997) 11:465-473.
  • 10[9]Kumar A.et al. , The Plant Journal (1996) 9:147-158.

共引文献156

同被引文献22

  • 1王俊英,王俊平,尹伟伦,夏新莉.人工调控植物基因表达研究[J].分子植物育种,2004,2(4):557-562. 被引量:2
  • 2Zhang Y,Zhao N.M,and Liu Q,The progress in antificial control system for gene expression,Chinese Science Bulletin 2000,45(10):865~870.
  • 3Clackson T.Regulated gene expression systems[J] Gene Therapy,2000,7:120~125.
  • 4Vogel R,Amarl,ThiAD,et al.A sigle lenticirus vector mediates doxycycline-regulated expression of transgenes in the brain[J] HumanGene Ther,2004,15(2):157~65.
  • 5Ohkawa J,Taira K.Control of the Functional Activity of an Antisense RNA by a Tetatracycline-Responsive Derivative of the Human U6 snRNA Promoter[J] Human Gene Therapy,2000,11:577~585.
  • 6Sung DY,Vierling E,Guy CL.Comprehensive expression profile analysis of Arabidopsis Hsp70 gene family.Plant Physiology,2001,126:789-800.
  • 7An G.Binary Ti vector for plant transformation and promoter analysis.Meth Enzymol,1987,153:293-305.
  • 8Horsch R B,Fry J E,Hoffman N L,et al.A simple and general method for transferring genes transferring genes into plants.Science,1985,227:1129~1131.
  • 9王关林,方宏筠.植物基因工程原理与技术(第2版).北京出版社,2000:805-806.
  • 10Jefferson R A.Assaying chimeric genes in plants:The gus gene fusion system.Plant Mol Biol Rep,1987,5:387~405.

引证文献4

二级引证文献10

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部