摘要
枯草芽孢杆菌是目前研究较为详尽的外源基因表达宿主,但由于受自身分泌蛋白酶多、构建的表达质粒不太稳定等因素影响,其应用受到一定的限制。文章综述了枯草芽孢杆菌基因组的特征、蛋白的分泌机制、重组表达质粒不稳定的原因,指出了枯草芽孢杆菌作为外源蛋白表达系统的瓶颈及今后的研究重点。
Recently, Bacillus subtilis as a valuable host for heterologous gene expression has been developed. It＇s application are restricted by secreting a variety of self-proteins and unstable recombination expression plasmids. This article has recounted the feature of genomie, protein secretion mechanisms, reasons of the recombination expression plasmids unstabilizing and point out the bottleneck as a expression system, along with the research orientation.
出处
《浙江农业学报》
CSCD
2005年第4期234-238,共5页
Acta Agriculturae Zhejiangensis
基金
浙江省重大攻关项目(2004C12035)
作者简介
沈卫锋（1977-），男，硕士，浙江桐乡人。主要从事昆虫分子生物学研究。
