摘要
目的体外观察抗坏血酸(ascroidacid,AA)对IL-1β诱导神经干细胞向酪氨酸羟化酶(tyrosinehydroxy-lase,TH)阳性神经元分化的作用,为帕金森病的细胞移植治疗提供实验资料。方法体外培养中脑神经干细胞并传代分组,在有血清条件下分别予以IL-1β及AA+IL-1β诱导分化,TH免疫细胞化学鉴定分化结果并细胞计数阳性比例。结果有血清条件下IL-1β、AA+IL-1β诱导中脑神经干细胞分化为TH阳性神经元比例约为12·6%和14·3%,明显高于对照组(约2·6%,P<0·01);两实验组间相对无统计学差异(P>0·05);联合诱导组TH阳性神经元细胞形态更成熟。结论IL-1β可以明显促进中脑神经干细胞分化为TH阳性神经元,AA能够加强IL-1β诱导的TH阳性细胞的表型成熟,但在我们的实验条件下二者联合未能增加TH阳性细胞诱导率。
Objective To investigate the effects of ascroid acid （AA） on the differentiation of mesencephalic neural stem cells （M-NSCs） into TH-positive （TH-ir） neurons induced by interleukin-1 bate （IL-1β） in vitro. Methods M-NSCs were cultured and passaged, then IL-1β and AA were used to induce differentiation in the presence of fetal bovine serum （FBS）. Immunocytochemical techniques and cell-count assessment were used to detect the TH-ir cells differenciated from NSCs. Results The proportion of MNSCs differentiated into TH-ir neurons was about 12. 6% and 14.3% in the IL-1β group and the AA＋IL-1β group respectively, which was much higer than that in the control group （2. 6%, P〈0. 01）. There were no statistical differences between the IL-1β group and AA＋IL-1β group, but the morphology of THir neurons were more mature in the latter than in the former group. Conclusion IL-1β can significantly increase the differentiation of TH-ir neurons. AA＋IL-1β can enhance the mature phenotype of TH-ir cells, but the combination did not increase the rate of production of TH-ir cells under our experimental conditions.
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
2005年第4期448-452,共5页
Chinese Journal of Histochemistry and Cytochemistry
基金
湖北省科技攻关课题资助(2002AB00149)
作者简介
彭超华，男（1979年），汉族，硕士研究生。
通讯作者（To whomcorre spondenceshould be addressed）
