摘要
目的分析本院2008～2009年常见细菌的分布及耐药性特点,为临床抗感染治疗提供参考依据。方法收集2008年1月～2009年12月本院临床标本中分离的居总数前8位的细菌菌株,利用全自动微生物鉴定仪V ITEK-32进行细菌鉴定及药敏实验,少数细菌药敏实验采用K-B法。结果 2年共分离病原菌1 667株。其中2008年共检出825株,2009年检出842株。常见细菌在本院检出总数及比例变化不明显。革兰阴性杆菌在各年度检出率均明显高于革兰阳性球菌,约占总菌株数的50%～60%。假铜绿单胞菌、大肠埃希菌、阴沟肠杆菌、肺炎克雷伯菌、醋酸钙鲍曼不动杆菌对亚胺培南、美罗培南、头孢哌酮/舒巴坦的敏感性高,阿米卡星对前4种细菌敏感性较高,哌拉西林/三唑巴坦对大肠、肺炎克雷伯菌敏感性较高,但这5种常见阴性杆菌对头孢类耐药率均超过50%。常见的革兰阳性球菌均对呋喃妥因、利奈唑烷、万古霉素敏感性很高。其中,万古霉素对这三种细菌中极个别细菌耐药,对青霉素几乎均耐药。结论本院细菌耐药性较高,应加强抗菌药物的合理应用,防止细菌耐药的蔓延。
Objective To analyse the common bacterial distribution and drug resistance characteristic in our hospital from 2008 to 2009 in order to provide the reference for clinical anti-infection treatment.Methods Bacterial strains from January 2008 to December 2009 which were isolated from our clinical samples accounted for the first eight of the total bacterial strains were collect.Automatic microbe instrument VITEK-32 for bacterial identification and drug susceptibility experiment were used.K-B disc method of a few bacteria antimicrobial susceptibility was adopted.Results 1 667 strains pathogens were isolated in two years.825 strains were detected in 2008 and 842 strains were detected in 2009.The total number and proportion of common bacteria in our hospital changed insignificantly.The annual detection rate of Gram-negative bacteria were higher than Gram-positie bacteria,the proportion of Gram-negative bacteria fluctuated around 50%～60%.The antimicrobial susceptibility of Pseudomonas aer uginosa,Escherichia coli,Enterobacter cloacae,Klebsiella pneumoniae,Calcium acetate complex Acinetobacter Baumann to imipenem,meropenem,cefoperazone / sulbactam was high.Pseudomonas aer uginosa,Escherichia coli,Enterobacter cloacae,Klebsiella pneumoniae were sensitive to Amikacin.The susceptibility of Piperacillin / tazobactam to Escherichia coli and Klebsiella pneumoniae was high,but the resistant rates of these five common negative bacillus to cephalosporins were more than 50%.Common Gram-positive cocci were sensitive to nitrofurantoin,rina thiazole cyclodienylsilane,vancomycin sensitivity.Among them,the resistance of vancomycin to extremely individual bacterial was high,but all bacterias were resistant to penicillin.Conclusion The bacteria resistant to antibiotic in our hospital was serious.We should strengthen the rational use of antibiotics to prevent the spread of bacteria resistance.
出处
《临床输血与检验》
CAS
2011年第3期228-230,共3页
Journal of Clinical Transfusion and Laboratory Medicine
关键词
菌群分布
药敏实验
耐药性
Flora distribution Medicine sensitive experiment Drug resistance
